FluoReporter™ Blue Fluorometric dsDNA Quantitation Kit, 200-2,000 assays - FAQs

Can I use Hoechst dyes to do both DNA quantitation and cell number counting in plates?

Yes, this can be done in a 96-well plate-based format. Hoechst dyes will label all cells, live or dead (or even fixed). This requires the use of a standard curve of known cell numbers (up to 100,000 cells per well) and a standard curve using purified DNA, , up to 10 µg/mL. We offer a kit for this: FluoReporter Blue Fluorometric dsDNA Quantitation Kit, Cat. No. F2962.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.