FluoReporter™ Biotin Quantitation Assay Kit, for biotinylated proteins
FluoReporter™ Biotin Quantitation Assay Kit, for biotinylated proteins
Invitrogen™

FluoReporter™ Biotin Quantitation Assay Kit, for biotinylated proteins

The FluoReporter™ Biotin Quantitation Assay Kit provides a sensitive fluorometric assay for determining the number of biotin labels on nucleicRead more
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Catalog NumberQuantity
F307511 Kit
Catalog number F30751
Price (USD)
287.65
Online Exclusive
383.00
Save 95.35 (25%)
Each
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Quantity:
1 Kit
Price (USD)
287.65
Online Exclusive
383.00
Save 95.35 (25%)
Each
Add to cart
The FluoReporter™ Biotin Quantitation Assay Kit provides a sensitive fluorometric assay for determining the number of biotin labels on nucleic acid samples (i.e., cDNA). The assay uses Biotective™ Green reagent, which contains a fluorescent dye that is quenched by ligands occupying the biotin binding sites. In the presence of biotin, the quencher dye ligands are displaced and fluorescence occurs. The fluorescence is proportional to the amount of added biotin. The results can be read with any fluorescence-based microplate reader capable of detecting FITC dye.

Important Features of FluoReporter™ Biotin Quantitation Assay Kits:
• Can detect from 4 to 80 picomoles of biotin in a sample (50-fold higher sensitivity than HABA biotin-binding assay)
• Can be applied to as little as 13 ng of biotin-labeled nucleic acid (more is needed for lower degrees of labeling)
• Excitation/emission maxima of the reagent is 495/519 nm
• Supplied with a biotinylated positive control for standard curve


For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
Product LineFluoReporter™
Product TypeBiotin Quantitation Assay Kit
Quantity1 Kit
Shipping ConditionRoom Temperature
Labeling TargetProteins
Label or DyeBiotin
Unit SizeEach
Contents & Storage
Store in freezer (-5 to -30°C) and protect from light.