FISH Tag™ RNA Far Red Kit with Alexa Fluor® 647 dye 10 reactions DISCONTINUED - FAQs

View additional product information for FISH Tag™ RNA Far Red Kit *with Alexa Fluor® 647 dye* *10 reactions* *DISCONTINUED* - FAQs (F32955)

2 product FAQs found

For imaging, what are the limitations of FISH probes?

FISH probes are generally from 300 to 1500 bp in length. To adequately image a gene or other target, the FISH probes must cover a contiguous length of ˜10 kbp. Shorter strand lengths or low-abundance DNA or RNA targets may be detected with the use of fluorescent amplification methods, such as tyramide signal amplification (TSA). For more information using TSA to amplify FISH detection, see this manual (http://tools.thermofisher.com/content/sfs/manuals/mp20912.pdf).

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When should I use an RNA FISH probe versus a DNA FISH probe?

RNA FISH probes are the best option for hybridization to mRNA, rRNA, or other RNA targets. DNA FISH probes can also be used to detect RNA targets, but they do not hybridize as well to mRNA and other RNA target molecules. DNA FISH probes are routinely used to detect gene loci on chromosomes. RNA FISH probes can also be used to detect genes on chromosomes, but DNA FISH probes are more robust for this application.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.