Novel beta-lactamase-random peptide fusion libraries for phage display selection of cancer cell-targeting agents suitable for enzyme prodrug therapy.
AuthorsShukla GS, Krag DN,
JournalJ Drug Target
PubMed ID19751096
'Novel phage-displayed random linear dodecapeptide (X(12)) and cysteine-constrained decapeptide (CX(10)C) libraries constructed in fusion to the amino-terminus of P99 beta-lactamase molecules were used for identifying beta-lactamase-linked cancer cell-specific ligands. The size and quality of both libraries were comparable to the standards of other reported phage display systems. Using the single-round ... More
Fluorogenic cephalosporin substrates for ß-lactamase TEM-1.
AuthorsRukavishnikov A, Gee KR, Johnson I, Corry S,
JournalAnal Biochem
PubMed ID21867672
'Cephalosporin was used to synthesize soluble and precipitating fluorogenic ß-lactam substrates that demonstrated differential catalytic hydrolysis by three different subtypes of ß-lactamase: TEM-1 (class A), p99 (class C), and a Bacillus cereus enzyme sold by Genzyme (class B). The most successful soluble substrate contained difluorofluorescein (Oregon Green 488) ligated to ... More
Cancer cell-specific internalizing ligands from phage displayed beta-lactamase-peptide fusion libraries.
AuthorsShukla GS, Krag DN,
JournalProtein Eng Des Sel
PubMed ID20219829
The present study was focused on identifying cancer cell-specific internalizing ligands using a new kind of phage display library in which the linear or cysteine-constrained random peptides were at amino-terminus fusion to catalytically active P99 beta-lactamase molecules. The size and quality of libraries were comparable to other reported phage display ... More
Phage-displayed combinatorial peptide libraries in fusion to beta-lactamase as reporter for an accelerated clone screening: Potential uses of selected enzyme-linked affinity reagents in downstream applications.
AuthorsShukla GS, Krag DN,
JournalComb Chem High Throughput Screen
PubMed ID20214576
Phage-display selection of combinatorial libraries is a powerful technique for identifying binding ligands against desired targets. Evaluation of target binding capacity of multiple clones recovered from phage display selection to a specific target is laborious, time-consuming, and a rate-limiting step. We constructed phage-display combinatorial peptide libraries in fusion with a ... More