FungaLight™ Yeast CFDA, AM/Propidium Iodide Vitality Kit

Catalog number: F34953

Molecular Probes™  Related applications: Cell Viability, Proliferation & Function | Flow Cytometry | Microbiological Analysis

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The FungaLight™ Yeast CFDA, AM / Propidium Iodide Vitality Kit combines a cell permeable esterase substrate with a membrane integrity indicator to evaluate the vitality of yeast cells by flow cytometry or microscopy. The acetoxymethyl ester (AM) of the esterase substrate 5-carboxyfluorescein diacetate (CFDA) allows this reagent to permeant cell membranes Once inside the cell, the lipophilic blocking and diacetate groups are cleaved by nonspecific esterases, resulting in a fluorescent, charged form that leaks out of cells very slowly. In contrast, the membrane integrity indicator, propidium iodide penetrates yeast with damaged membranes. With an appropriate mixture of the CFDA, AM and propidium iodide stains, esterase-active yeast with intact cell membranes stain fluorescent green, whereas yeast with damaged membranes stain fluorescent red.
For Research Use Only. Not for use in diagnostic procedures.


Form: Solid
Format: Slide(s), Tube(s)
Label or Dye: CFDA, propidium iodide
Product Size: 1 kit
Compatible Cells: Yeast Cells
Detection Method: Fluorescent
Number of Reactions: 200
For Use With (Equipment): Fluorescence Microscope, Flow Cytometer
Excitation⁄Emission (nm): CFDA: 492⁄517, PI: 490⁄635
Shipping Condition: Room Temperature

Contents & storage

Contains 3 vials of 5-CFDA (1 mg per vial), 1 vial of propidium iodide, and 1 vial of DMSO.

Store in freezer (-5 to -30°C) and protect from light.


Manuals & protocols

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