FungaLight™ Yeast CFDA, AM/Propidium Iodide Vitality Kit

Catalog number: F34953

Molecular Probes™  Related applications: Cell Viability, Proliferation & Function | Flow Cytometry | Microbiological Analysis

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

The FungaLight™ Yeast CFDA, AM / Propidium Iodide Vitality Kit combines a cell permeable esterase substrate with a membrane integrity indicator to evaluate the vitality of yeast cells by flow cytometry or microscopy. The acetoxymethyl ester (AM) of the esterase substrate 5-carboxyfluorescein diacetate (CFDA) allows this reagent to permeant cell membranes Once inside the cell, the lipophilic blocking and diacetate groups are cleaved by nonspecific esterases, resulting in a fluorescent, charged form that leaks out of cells very slowly. In contrast, the membrane integrity indicator, propidium iodide penetrates yeast with damaged membranes. With an appropriate mixture of the CFDA, AM and propidium iodide stains, esterase-active yeast with intact cell membranes stain fluorescent green, whereas yeast with damaged membranes stain fluorescent red.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Compatible Cells: Yeast Cells
Detection Method: Fluorescent
Excitation⁄Emission (nm): CFDA: 492⁄517, PI: 490⁄635
For Use With (Equipment): Flow Cytometer, Fluorescence Microscope
Form: Solid
Format: Slide(s), Tube(s)
Label or Dye: CFDA, propidium iodide
Number of Reactions: 200
Product Size: 1 kit
Shipping Condition: Room Temperature

Contents & storage

Contains 3 vials of 5-CFDA (1 mg per vial), 1 vial of propidium iodide, and 1 vial of DMSO.

Store in freezer (-5 to -30°C) and protect from light.

Documents

Manuals & protocols

Recommended products