Invitrogen™

Fluo-4 NW Calcium Assay Kit

Catalog number: F36206
Invitrogen™

Fluo-4 NW Calcium Assay Kit

Catalog number: F36206
Catalog Number
F36206
Unit Size
10 microplates
Price (USD)
298.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
100 microplates
Price (USD)
2,195.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
F3620610 microplates
298.00
Your Price:
***
F36205100 microplates
2,195.00
Your Price:
***
Product Overview
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Fluo-4 NW (No-Wash) Calcium Assay Kitsoffer a proprietary calcium assay formulation that requires neither a washstep nor a quencher dye. The fluo-4 NW assay achieves largerincreases in fluorescence intensity than standard fluo-3 and fluo-4assays with a wash step. Eliminating the wash step results inlower variability and higher Z´ values than the standard fluo-4 assay,while providing an easier and faster assay as well.

The fluo-4NW indicator is nonfluorescent and stable in pH 7–7.5 buffer forseveral hours, so spontaneous conversion to the Ca2+-sensitive formis not a significant source of background fluorescence. Contributionsto baseline fluorescence by the growth medium (e.g., esteraseactivity, proteins interacting with receptors of interest, or phenolred) are eliminated by removing the medium prior to adding theindicator dye to the wells.

Another source of potential fluorescenceoutside the cells is extrusion of the indicator out of the cell by organicanion transporters. Probenecid is commonly used to inhibitthis transport and reduce the baseline signal. We have synthesizeda proprietary water-soluble probenecid, which is supplied with theFluo-4 NW Calcium Assay Kits. This form of probenecid has theadvantages of being easy to dissolve in buffer and safer to use thanthe free acid, which requires caustic 1 M NaOH to dissolve. TheFluo-4 NW Calcium Assay Kits are designed for microplates andHTS, and the assay can be performed on adherent as well as nonadherentcells.

Fluo-4 AM is a fluorescent Ca+2 indicator that is widely usedfor in-cell measurement of agonist-stimulated and antagonist inhibitedcalcium signaling in high-throughput screening (HTS)applications. Its visible wavelength excitation (compatible withargon-ion laser sources), high sensitivity, and large fluorescenceincrease upon binding Ca2+ has made it the indicator of choicefor characterizing G-protein–coupled receptor (GPCR) pharmacologyand function. These properties have made fluo-4 AMattractive not only for microplate screening applications but formicroscopy and flow cytometry as well.

WARNING: Cancer and Reproductive Harm – www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method
Fluorescent
Dye Type
Fluorescent Dye-Based
Quantity
10 Microplate(s)
Shipping Condition
Room Temperature
For Use With (Equipment)
Microplate Reader

Contents & Storage

Store in freezer -5°C to -30°C and protect from light.

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