DyNAmo ColorFlash SYBR Green qPCR Kit

Catalog number: F416S

Thermo Scientific™  Related applications: Real Time PCR (qPCR)

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Description

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Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of DyNAmo ColorFlash SYBR Green qPCR Kit and added additional capabilities for your gene expression analysis.

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Thermo Scientific DyNAmo ColorFlash SYBR Green qPCR Kit offers equal performance to the DyNAmo Flash SYBR Green qPCR Kit. In addition, it incorporates an innovative multi-color system that ensures correct pipetting. The 2X master mix contains a blue dye, and a separate sample buffer contains a yellow dye. The qPCR reaction mix containing both components is green. Using this patent-pending multicolor system, pipetting of both the master mix and the sample can be easily monitored. This significantly decreases the risk for pipetting errors during reaction setup, especially when using white reaction vessels. The dyes do not affect the specificity or sensitivity of qPCR assays.

Highlights

• Colored reagents minimize the risk of pipetting errors during reaction set up
• Especially beneficial when using white reaction vessels
• Extremely fast protocols due to combined annealing and extension step of only 15 s
• Specific and sensitive detection of a wide range of template concentrations
• dUTP included in the 2x master mix allows the use of UNG for prevention of carry-over contamination

Applications

• Fast qPCR
• qPCR using SYBR Green dye
• RT-qPCR using SYBR Green dye

Related Products
DyNamo ColorFlash Probe qPCR Kit
DyNAmo ColorFlash SYBR Green qPCR Kit
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: SYBR
For Use With (Equipment): 5700 Sequence Detection System, 7000 System, 7300 System, 7500 Fast System, 7500 System, 7700 System, 7900HT Fast System, 7900HT System, Bio-Rad CFX384 Touch™, BioRad CFX96™, Cepheid SmartCycler, Corbett RotorGene, Eppendorf MasterCycler RealPlex, MJ Chromo4, MJ Opticon, QuantStudio™, Roche LightCycler 480, StepOne™ System, StepOnePlus™ System, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, ViiA™ 7 System
Reaction Speed: Fast
PCR Method: qPCR
Sample Type (General): DNA, dsDNA
Number of Reactions: 100 reactions
Product Line: DyNAmo Flash
Product Size: 100 reactions

Contents & storage

Contains:
• 1 x 1 mL DyNAmo ColorFlash SYBR Green qPCR Mix (2X)
• 1 x 50 µL 50X ROX dye, 25µM
• 1 x 200 µL 40x sample buffer solution with yellow dye

Store at -20°C

Documents

Manuals & protocols

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