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DyNAmo Flash Probe qPCR Kit

Catalog number: F455L

Thermo Scientific™  Related applications: Real Time PCR (qPCR)

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Description

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Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of DyNAmo Flash Probe qPCR Kit and added additional capabilities for your gene expression analysis.

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Thermo Scientific DyNAmo Flash Probe qPCR Kit is the perfect choice for fast probe-based qPCR. The optimized reagents and extremely short cycling times deliver maximum speed thus increasing sample throughput. Significant time savings are achieved with both conventional and fast real-time PCR instruments. Performance of the DyNAmo Flash Probe qPCR Kit is based on an efficient hot start Thermus brockianus DNA polymerase. Sensitivity and the excellent amplification efficiency across a wide range of template concentrations guarantee reproducible results.

DyNAmo Flash Probe qPCR Kit is optimized for hydrolysis probes (e.g., TaqMan probes) and it can be used with both block- and capillary-based realtime PCR instruments.

Highlights

• Extremely fast protocols: combined annealing and extension step of only 15 s
• Specific and sensitive detection of a wide range of template concentrations
• Included dUTP allows the use of UNG for prevention of carry-over contamination
• Convenient 2X master mix and optimized protocols

Applications

• Fast qPCR
• qPCR using sequence-specific probes
• RT-qPCR using sequence-specific probes

Related Products
DyNAmo Flash Probe qPCR Kit
DyNAmo Flash SYBR Green qPCR Kit
DyNAmo HS SYBR Green qPCR Kit
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: Primer-Probe Detection
For Use With (Equipment): 5700 Sequence Detection System, 7000 System, 7300 System, 7500 Fast System, 7500 System, 7700 System, 7900HT Fast System, 7900HT System, Bio-Rad CFX384 Touch™, BioRad CFX96™, BioRad MyiQ, BioRad iCycler iQ, BioRad iQ5, Cepheid SmartCycler, Corbett RotorGene, Eppendorf® MasterCycler® RealPlex, MJ Chromo4, MJ Opticon, QuantStudio™, Roche LightCycler 480, StepOne™ System, StepOnePlus™ System, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, ViiA™ 7 System
Reaction Speed: Fast
PCR Method: qPCR
Sample Type (General): DNA, dsDNA
Number of Reactions: 500 reactions
Product Line: DyNAmo Flash
Product Size: 500 reactions

Contents & storage

Contains:
• 5 x 1 mL DyNAmo Flash qPCR Mix (2X)
• 1 x 250 µL 50X ROX dye, 25µM

Store at -20°C

Documents

Manuals & protocols