DyNamo ColorFlash Probe qPCR Kit

Catalog number: F456L

Thermo Scientific™  Related applications: Real Time PCR (qPCR)

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Thermo Scientific DyNAmo ColorFlash Probe qPCR Kit offers equal performance to the DyNAmo Flash Probe qPCR Kit. In addition, it incorporates an innovative multicolor system that ensures correct pipetting. The 2X master mix contains a blue dye, and a separate sample buffer contains a yellow dye. The qPCR reaction mix containing both components is green. Using this patent-pending multicolor system, pipetting of both the master mix and the sample can be easily monitored. This significantly decreases the risk for pipetting errors during reaction set up, especially when using white reaction vessels. The dyes do not affect the specificity or sensitivity of qPCR assays.


• Minimized risk of pipetting errors during reaction set up
• Especially beneficial when using white reaction vessels
• Extremely fast protocols due to combined annealing and extension step of only 15 s
• Specific and sensitive detection of a wide range of template concentrations
• dUTP included in the 2X master mix allows the use of UNG for prevention of carry-over contamination


• Fast qPCR
• qPCR using sequence-specific probes
• RT-qPCR using sequence-specific probes

Related Products
DyNamo ColorFlash Probe qPCR Kit
DyNAmo ColorFlash SYBR Green qPCR Kit
For Research Use Only. Not for use in diagnostic procedures.


Detection Method: Primer-Probe Detection
For Use With (Equipment): 7300 System, 7700 System, Cepheid SmartCycler, BioRad iCycler iQ, BioRad MyiQ, MJ Opticon, Stratagene Mx3005P, 5700 Sequence Detection System, Roche LightCycler 480, Bio-Rad CFX384 Touch™, ViiA™ 7 System, BioRad CFX96™, Corbett RotorGene, 7900HT Fast System, 7500 Fast System, Stratagene Mx4000, StepOne™ System, Eppendorf MasterCycler RealPlex, QuantStudio™, StepOnePlus™ System, Stratagene Mx3000P, MJ Chromo4, BioRad iQ5, 7500 System, 7900HT System, 7000 System
Reaction Speed: Fast
PCR Method: qPCR
Sample Type (General): dsDNA, DNA
Product Size: 500 reactions
Number of Reactions: 500 reactions

Contents & storage

• 5 x 1 mL DyNAmo ColorFlash qPCR Mix (2X)
• 1 x 250 µL 50X ROX dye, 25µM
• 1 x 1000 µL 40X sample buffer solution with yellow dye

Store at -20°C


Manuals & protocols