Error:Error:As of December 31, 2021, this product will be discontinued and will remain in stock until sold out. We recommend TaqMan™ Fast Advanced Master Mix—our newest and best performing RUO TaqMan master mix.
Thermo Scientific™

DyNamo ColorFlash Probe qPCR Kit

Catalog number: F456L
Thermo Scientific™

DyNamo ColorFlash Probe qPCR Kit

Catalog number: F456L
Catalog Number
F456L
also known as F-456L
Unit Size
500 reactions
Price (USD)
472.00
Your Price:
Availability
***
Quantity
Catalog Number
F456XL
also known as F-456XL
Unit Size
2,500 reactions
Price (USD)
1,942.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
F456L
also known as F-456L
500 reactions
472.00
Your Price:
***
F456XL
also known as F-456XL
2,500 reactions
1,942.00
Your Price:
***
Product Overview
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Additional Information
Recommendations
Recommendations
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Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of DyNamo ColorFlash Probe qPCR Kit and added additional capabilities for your gene expression analysis.

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Thermo Scientific DyNAmo ColorFlash Probe qPCR Kit offers equal performance to the DyNAmo Flash Probe qPCR Kit. In addition, it incorporates an innovative multicolor system that ensures correct pipetting. The 2X master mix contains a blue dye, and a separate sample buffer contains a yellow dye. The qPCR reaction mix containing both components is green. Using this patent-pending multicolor system, pipetting of both the master mix and the sample can be easily monitored. This significantly decreases the risk for pipetting errors during reaction set up, especially when using white reaction vessels. The dyes do not affect the specificity or sensitivity of qPCR assays.

Highlights

• Minimized risk of pipetting errors during reaction set up
• Especially beneficial when using white reaction vessels
• Extremely fast protocols due to combined annealing and extension step of only 15 s
• Specific and sensitive detection of a wide range of template concentrations
• dUTP included in the 2X master mix allows the use of UNG for prevention of carry-over contamination

Applications

• Fast qPCR
• qPCR using sequence-specific probes
• RT-qPCR using sequence-specific probes

Related Products
DyNamo ColorFlash Probe qPCR Kit
DyNAmo ColorFlash SYBR Green qPCR Kit

Specifications

Concentration
2X (DyNAmo ColorFlash qPCR Mix), 50X (ROX Dye), 40X (Sample Buffer Solution with Yellow Dye)
Detection Method
Primer-Probe Detection
Format
Tube
GC-Rich PCR Performance
High
No. of Reactions
500 reactions
PCR Method
qPCR
Polymerase
Tbr DNA polymerase
Reaction Speed
Fast
Sample Type
DNA, dsDNA
Volume
5 x 1 mL
For Use With (Application)
Real Time PCR (qPCR)
For Use With (Equipment)
7000 System, 7300 System, 7500 Fast System, 7500 System, 7700 System, 7900HT Fast System, 7900HT System, BioRad CFX96™, QuantStudio™, ViiA™ 7 System, Bio-Rad CFX384 Touch™, Roche LightCycler 480, 5700 Sequence Detection System, StepOnePlus™ System, Eppendorf MasterCycler RealPlex, Corbett RotorGene, Stratagene Mx4000, StepOne™ System, Stratagene Mx3000P, MJ Opticon, MJ Chromo4, BioRad MyiQ, BioRad iQ5, BioRad iCycler iQ, Cepheid SmartCycler, Stratagene Mx3005P
Kit Type
DyNAmo ColorFlash Probe qPCR Kit
Product Line
DyNAmo Flash
Product Type
Real Time PCR SYBR Master Mix
Sufficient For
500 reactions of 20 μL, 200 reactions of 50 μL

Contents & Storage

Contains:
• 5 x 1 mL DyNAmo ColorFlash qPCR Mix (2X)
• 1 x 250 µL 50X ROX dye, 25µM
• 1 x 1000 µL 40X sample buffer solution with yellow dye

Store at -20°C

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