DyNamo ColorFlash Probe qPCR Kit

Catalog number: F456XL

Thermo Scientific™  Related applications: Real Time PCR (qPCR)

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Thermo Scientific DyNAmo ColorFlash Probe qPCR Kit offers equal performance to the DyNAmo Flash Probe qPCR Kit. In addition, it incorporates an innovative multicolor system that ensures correct pipetting. The 2X master mix contains a blue dye, and a separate sample buffer contains a yellow dye. The qPCR reaction mix containing both components is green. Using this patent-pending multicolor system, pipetting of both the master mix and the sample can be easily monitored. This significantly decreases the risk for pipetting errors during reaction set up, especially when using white reaction vessels. The dyes do not affect the specificity or sensitivity of qPCR assays.


• Minimized risk of pipetting errors during reaction set up
• Especially beneficial when using white reaction vessels
• Extremely fast protocols due to combined annealing and extension step of only 15 s
• Specific and sensitive detection of a wide range of template concentrations
• dUTP included in the 2X master mix allows the use of UNG for prevention of carry-over contamination


• Fast qPCR
• qPCR using sequence-specific probes
• RT-qPCR using sequence-specific probes

Related Products
DyNamo ColorFlash Probe qPCR Kit
DyNAmo ColorFlash SYBR Green qPCR Kit
For Research Use Only. Not for use in diagnostic procedures.


Detection Method: Primer-Probe Detection
For Use With (Equipment): BioRad CFX96™, Bio-Rad CFX384 Touch™, 5700 Sequence Detection System, QuantStudio™, StepOne™ System, 7000 System, 7900HT System, Stratagene Mx3005P, Stratagene Mx3000P, MJ Opticon, MJ Chromo4, BioRad MyiQ, BioRad iQ5, BioRad iCycler iQ, 7500 System, Cepheid SmartCycler, Corbett RotorGene, 7900HT Fast System, 7500 Fast System, Stratagene Mx4000, 7700 System, 7300 System, Eppendorf MasterCycler RealPlex, StepOnePlus™ System, Roche LightCycler 480, ViiA™ 7 System
Reaction Speed: Fast
PCR Method: qPCR
Sample Type (General): dsDNA, DNA
Product Size: 2,500 reactions
Number of Reactions: 2500 reactions

Contents & storage

• 25 x 1 mL DyNAmo ColorFlash qPCR Mix (2X)
• 1 x 1.25 mL 50X ROX dye (25µM)
• 5 x 1 mL 40X sample buffer solution with yellow dye

Store at -20°C


Manuals & protocols