Phusion™ HF and GC Buffer Packs
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Thermo Scientific™

Phusion™ HF and GC Buffer Packs

5X Phusion Green buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel.
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Catalog NumberProduct TypeColor
F518LHF Buffer PackColorless
F519LGC Buffer PackColorless
F520LDetergent-free FF Buffer PackColorless
F521LDetergent-free GC Buffer PackColorless
F538LHF Buffer PackGreen
F539LGC Buffer PackGreen
Catalog number F518L
Price (USD)/ Each
33.65
Online exclusive
37.07 
Save 3.42 (9%)
-
Add to cart
Product Type:
HF Buffer Pack
Color:
Colorless
Request bulk or custom format
Price (USD)/ Each
33.65
Online exclusive
37.07 
Save 3.42 (9%)
Add to cart
Phusion™ HF and GC Buffer Packs
Catalog numberF518L
Price (USD)/ Each
33.65
Online exclusive
37.07 
Save 3.42 (9%)
-
Add to cart
The error rate of Phusion DNA Polymerase in HF Buffer (4.4 x 10-7) is lower than that in GC Buffer (9.5 x 10-7). Therefore, the HF Buffer should be used as the default buffer for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures. For applications such as microarray or DHPLC, where the DNA templates need to be free of detergents, we recommend using detergent-free reaction buffers.

5X Phusion Green Buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffers do not interfere with the enzyme performance and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Two optimized buffers are available for Thermo Scientific Phusion High-Fidelity DNA Polymerases:

  • 5X Phusion HF Buffer (F-518L, F-538L (Phusion Green), F-520L (Detergent-free))
  • 5X Phusion GC Buffer (F-519L, F-539L (Phusion Green), F-521L (Detergent-free))
For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptionPhusion™ HF Buffer Pack
PolymerasePhusion High-Fidelity DNA Polymerase
Product TypeHF Buffer Pack
Quantity4 x 1.5 mL
Shipping ConditionWet or Dry Ice
For Use With (Application)Standard PCR, High-fidelity PCR
Starting MaterialDNA
Volume4 x 1.5 mL
ColorColorless
Unit SizeEach
Contents & Storage
• 5X Phusion HF Reaction Buffer (4 x 1.5 mL)
• 50 mM MgCl2 (4 x 1.5 mL)
• DMSO (0.5 mL)

Store at –20°C.