Thermo Scientific™

Phusion GC Buffer Pack

Catalog number: F519L
Thermo Scientific™

Phusion GC Buffer Pack

Catalog number: F519L
Catalog Number
F519L
also known as F-519L
Unit Size
4 x 1.5 mL
Price (USD)
Price: 32.01
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
F519L
also known as F-519L
4 x 1.5 mL
Price: 32.01
Your Price:
***
Product Overview
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Additional Information
Recommendations
Recommendations
Two optimized buffers are available for Thermo Scientific Phusion High-Fidelity DNA Polymerases:

• 5X Phusion HF Buffer (F-518L, F-538L (Phusion Green), F-520L (Detergent-free))
• 5X Phusion GC Buffer (F-519L, F-539L (Phusion Green), F-521L (Detergent-free))

The error rate of Phusion DNA Polymerase in HF Buffer (4.4 × 10-7) is lower than that in GC Buffer (9.5 × 10-7). Therefore, the HF Buffer should be used as the default buffer for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures. For applications such as microarray or DHPLC, where the DNA templates need to be free of detergents, we recommend using detergent-free reaction buffers.

5X Phusion Green Buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffers do not interfere with the enzyme performance and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Related Products
Phusion GC Buffer Pack, detergent-free
Phusion HF Buffer Pack, detergent-free
Phusion HF Buffer Pack
Phusion Green HF Buffer Pack
Phusion Green GC Buffer Pack
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Application)
PCR Amplification
Description
Phusion™ GC Buffer Pack
Format
Kit
Polymerase
Phusion High-Fidelity DNA Polymerase
Starting Material
DNA
Volume
4 x 1.5 mL
Product Line
Phusion
Quantity
6 mL

Contents & Storage

Phusion GC Buffer Pack includes:
• 5X Phusion GC Buffer (1X buffer provides 1.5 mM MgCl2)
• 50 mM MgCl2
• DMSO

Store at -20°C.

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