Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Catalog Number | Product Type |
---|---|
F524L | Phire Reaction Buffer |
F527L | Phire Green Reaction Buffer |
F525L | Phire Reaction Buffer, detergent-free |
Phire Reaction Green Buffer includes a blue die that migrates with 3–5 kb DNA fragments in 1% agarose gel and a yellow dye that migrates faster than 10 bp DNA fragments in 1% agarose gel. The dyes have excitation peaks at 424 nm and 615 nm.
The concentration of Mg2+ in Phire Reaction Buffer is optimized to work well for most amplicons. The buffer contains 1.5 mM MgCl2 at final reaction concentration. Separate tubes of MgCl2 and DMSO are provided for further optimization.
Notes