Thermo Scientific™

Phire Green Reaction Buffer

Catalog number: F527L
Thermo Scientific™

Phire Green Reaction Buffer

Catalog number: F527L
Catalog Number
F527L
also known as F-527L
Unit Size
4 x 1.5 mL
Price (USD)
Price: 32.46
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
F527L
also known as F-527L
4 x 1.5 mL
Price: 32.46
Your Price:
***
Product Overview
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The reaction buffer for Thermo Scientific Phire Hot Start II DNA Polymerase is available in three formats: non-colored version with and without detergents (F-524L and F-525L, respectively) or Green version (F-527L) containing a density reagent and two tracking dyes for direct loading of PCR products on gel. The colored buffer does not interfere with enzyme performance and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

The concentration of Mg2+ in the reaction buffers is optimized to work well for most amplicons. The buffer contains 1.5 mM MgCl2 at final reaction concentration. Separate tubes of MgCl2 and DMSO are provided for further optimization.

Related Products
Phire Reaction Buffer
Phire Reaction Buffer, detergent-free
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration
5X
Polymerase
Phire Hot Start II DNA polymerase
Volume
4 x 1.5 mL
Product Line
Phire
Product Type
PCR Reaction Buffer

Contents & Storage

Contains:
• 4 x 1.5 mL 5X Phire Green Reaction Buffer (1X buffer provides 1.5 mM MgCl2)
• 50 mM MgCl2
• DMSO

Store at -20°C.

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