Phusion High-Fidelity PCR Master Mix with GC Buffer

Catalog number: F532L

Thermo Scientific™  Related applications: PCR

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Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq, and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity. Phusion DNA Polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerase.

Phusion High-Fidelity PCR Master Mix is convenient 2X mix containing Phusion DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2. Two master mix formulations are available - with HF Buffer (F-531S and F-531L) and with GC Buffer (F-532S and F-532L). The error rate of Phusion DNA Polymerase in HF Buffer (4.4 × 10-7) is lower than that in GC Buffer (9.5 × 10-7). Therefore, the master mix with HF Buffer should be used as the default master mix for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures.


• High fidelity (25X Taq)
• Fast PCR due to short extension times (15-30 s/kb)
• Robust performance, minimal optimization needed


• Amplification of difficult (GC-rich) templates
• High-fidelity PCR
• Cloning
• Template generation for sequencing
• Long-range PCR (up to 20 kb)
• Mutagenesis
• High throughput PCR
For Research Use Only. Not for use in diagnostic procedures.


Polymerase: Phusion High-Fidelity DNA Polymerase
Hot Start: No
Fidelity (vs. Taq): 25 X
GC-Rich PCR Performance: High
Reaction Format: SuperMix or Master Mix
Product Overhang: Blunt
Reaction Speed: Fast
Product Size: 500 reactions

Contents & storage

Contains Phusion DNA Polymerase, 400 µM of each dNTP and 2X Phusion GC Buffer (provides 1.5 mM MgCl2 in final reaction concentration). Supplied with separate vial of DMSO.

Store at -20°C.


Manuals & protocols

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