Thermo Scientific™

Phusion High-Fidelity PCR Master Mix with GC Buffer

Catalog number: F532L
Thermo Scientific™

Phusion High-Fidelity PCR Master Mix with GC Buffer

Catalog number: F532L
Catalog Number
F532L
also known as F-532L
Unit Size
500 reactions
Price (USD)
Price: 830.00
Your Price:
Availability
***
Quantity
Catalog Number
F532S
also known as F-532S
Unit Size
100 reactions
Price (USD)
Price: 204.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
F532L
also known as F-532L
500 reactions
Price: 830.00
Your Price:
***
F532S
also known as F-532S
100 reactions
Price: 204.00
Your Price:
***
Product Overview
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Additional Information
Recommendations
Recommendations
Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq, and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity. Phusion DNA Polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerase.

Phusion High-Fidelity PCR Master Mix is convenient 2X mix containing Phusion DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2. Two master mix formulations are available - with HF Buffer (F-531S and F-531L) and with GC Buffer (F-532S and F-532L). The error rate of Phusion DNA Polymerase in HF Buffer (4.4 × 10-7) is lower than that in GC Buffer (9.5 × 10-7). Therefore, the master mix with HF Buffer should be used as the default master mix for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures.

Highlights

• High fidelity (25X Taq)
• Fast PCR due to short extension times (15-30 s/kb)
• Robust performance, minimal optimization needed

Applications

• Amplification of difficult (GC-rich) templates
• High-fidelity PCR
• Cloning
• Template generation for sequencing
• Long-range PCR (up to 20 kb)
• Mutagenesis
• High throughput PCR

Using Phusion DNA Polymerases
Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our Tm calculator at www.thermofisher.com/tmcalculator.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Application)
PCR Amplification
Concentration
2X
Fidelity (vs. Taq)
25 X
Format
SuperMix or Master Mix
GC-Rich PCR Performance
High
Hot Start
No
No. of Reactions
500
Overhang
Blunt
Polymerase
Phusion High-Fidelity DNA Polymerase
Purity
DHPLC
Reaction Format
SuperMix or Master Mix
Reaction Speed
Fast
Size (Final Product)
20 kb or Less
Starting Material
DNA
Includes
With GC Buffer
Product Line
Phusion
Product Type
High-Fidelity PCR Master Mix
Quantity
500 x 50 μL Reactions

Contents & Storage

• 10 x 1.25 mL Phusion Master Mix with GC Buffer (2X) (provides 1.5 mM MgCl2 in final reaction)
• 2 x 500 μL 100% DMSO

Store at -20°C.

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