Thermo Scientific™

Phusion U Hot Start PCR Master Mix

Catalog number: F533L
Thermo Scientific™

Phusion U Hot Start PCR Master Mix

Catalog number: F533L
Catalog Number
F533L
also known as F-533L
Unit Size
500 reactions
Price (USD)
Price: 1,088.00
Your Price:
Availability
***
Quantity
Catalog Number
F533S
also known as F-533S
Unit Size
100 reactions
Price (USD)
Price: 277.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
F533L
also known as F-533L
500 reactions
Price: 1,088.00
Your Price:
***
F533S
also known as F-533S
100 reactions
Price: 277.00
Your Price:
***
Product Overview
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Thermo Scientific Phusion U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology. Due to a proprietary mutation in the so called dUTP binding pocket of Phusion, Phusion U overcomes an important limitation of proofreading enzymes - it is able to incorporate dUTP and read through uracil present in DNA templates.

In addition to uracil usage possibility, Phusion U features all the superior properties of other Phusion DNA Polymerases - great accuracy, speed, ability to amplify long amplicons up to 20 kb and a high specificity with Affibody based hot start. These features make Phusion U Hot Start DNA Polymerase an excellent choice for such important applications as amplification of bisulphite-converted or damaged DNA as well as use of carryover contamination control.

Phusion U Hot Start PCR Master Mix is convenient 2X mix containing Phusion U Hot Start DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2. Only template and primers need to be added by the user.

Highlights

Accuracy—high fidelity DNA polymerase (25x Taq)
Uracil-tolerance —engineered to incorporate dUTP and amplify uracil containing templates
Specificity—hot start for reduced nonspecific amplification and primer degradation
Speed —short extension times (15-30 s/kb)

Applications

• Amplification of bisulphite-converted DNA
• Amplification of damaged or aged DNA
• Carry-over contamination control
• Uracil-excision based (USER) cloning methods

Using Phusion DNA Polymerases
Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our Tm calculator at www.thermofisher.com/tmcalculator.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration
2X
Fidelity (vs. Taq)
25 X
Format
SuperMix or Master Mix
GC-Rich PCR Performance
Medium
Hot Start
Built-In Hot Start
No. of Reactions
500 x 50 mL Reactions
Overhang
Blunt
Polymerase
Phusion U Hot Start DNA Polymerase
Reaction Format
SuperMix or Master Mix
Reaction Speed
Fast
Size (Final Product)
20 kb
Starting Material
DNA
Product Line
Phusion
Product Type
Hot Start PCR Master Mix
Quantity
500 x 50 μL Reactions
Sufficient For
500 x 50 μL Reactions

Contents & Storage

Phusion U Hot Start PCR Master Mix includes:
• Phusion U Hot Start DNA Polymerase
• 2X reaction buffer
• 3 mM MgCl2
• 400 µM of each dATP, dTTP, dCTP and dGTP
The master mix is supplied with nuclease-free water.
Store at -20°C.

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