Informational:Inquire about OEM or Commercial Supply version of this product here.
Thermo Scientific™

Phusion Green High-Fidelity DNA Polymerase (2 U/μL)

Catalog number: F534L
Thermo Scientific™

Phusion Green High-Fidelity DNA Polymerase (2 U/μL)

Catalog number: F534L
Catalog Number
F534L
also known as F-534L
Unit Size
500 units
Price (USD)
Price: 540.00
Online price: 372.65
Your price:
Availability
-
Quantity
Catalog Number
Unit Size
100 units
Price (USD)
Price: 134.00
Online price: 120.65
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
F534L
also known as F-534L
500 units
Price: 540.00
Online price: 372.65
Your price:
-
F534S100 units
Price: 134.00
Online price: 120.65
Your price:
-
Product Overview
Figures
Videos
Recommendations
Recommendations
Documents
FAQ
Citations & References
Additional Information
Recommendations

Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq, and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity. Phusion DNA Polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerase.

The Phusion Green format is a combination of Phusion High-Fidelity DNA Polymerase with 5X Phusion Green Buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The density reagent and dyes do not interfere with excellent performance of Phusion DNA Polymerases and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Highlights
• High fidelity (52X Taq)
• Fast PCR due to short extension times (15-30 s/kb)
• Robust performance, minimal optimization needed
• High yields of PCR products with minimal enzyme amounts
• Available as a Green buffer format for direct loading of PCR products on gels (F-534S or F-534L)

Applications
• High-fidelity PCR
• Cloning
• Template generation for sequencing
• Amplification of difficult (GC-rich) templates
• Long-range PCR (up to 20 kb)
• Mutagenesis
• High throughput PCR
• Microarray

Using Phusion DNA Polymerases
Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our Tm calculator at www.thermofisher.com/tmcalculator.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

GC-Rich PCR Performance
High
Polymerase
Phusion High-Fidelity DNA Polymerase
Reaction Speed
Fast
Product Type
High-Fidelity DNA Polymerase
Quantity
500 U
Shipping Condition
Dry Ice
For Use With (Application)
Standard PCR, High-fidelity PCR
Concentration
2 U/μL
Fidelity (vs. Taq)
52X
Hot Start
No
No. of Reactions
500 Reactions
Overhang
Blunt
Reaction Format
Standalone
Size (Final Product)
20 kb or less

Contents & Storage

• Phusion DNA Polymerase, 2 U/μL
• 5X Phusion Green HF and GF Buffers
• DMSO
• 50 mM MgCl2

Phusion Green HF and Phusion Green GC Buffers each provide 1.5 mM MgCl2 in the final 1X concentration.
Store at -20°C.

Figures

Documents & Downloads

Certificates

    Frequently asked questions (FAQs)

    Citations & References

    Search citations by name, author, journal title or abstract text