Phusion Site-Directed Mutagenesis Kit
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Phusion Site-Directed Mutagenesis Kit
Thermo Scientific™

Phusion Site-Directed Mutagenesis Kit

Thermo Scientific Phusion Site-Directed Mutagenesis Kit is a versatile and efficient tool for introducing point mutations, insertions, or deletions inRead more
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Catalog number F541
Price (USD)/ 20 reactions
197.65
Online exclusive
220.00 
Save 22.35 (10%)
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Add to cart
Request bulk or custom format
Price (USD)/ 20 reactions
197.65
Online exclusive
220.00 
Save 22.35 (10%)
Add to cart
Phusion Site-Directed Mutagenesis Kit
Catalog numberF541
Price (USD)/ 20 reactions
197.65
Online exclusive
220.00 
Save 22.35 (10%)
-
Add to cart
Thermo Scientific Phusion Site-Directed Mutagenesis Kit is a versatile and efficient tool for introducing point mutations, insertions, or deletions in any type of plasmid DNA. With this kit, the entire plasmid is amplified using phosphorylated primers that introduce the desired changes. The amplified, linear PCR product, containing the desired mutation, is circularized in a 5-minute ligation reaction with T4 DNA Ligase. The resulting plasmid can be then transformed into any competent E. coli cells.

The optimal annealing temperature for Phusion DNA Polymerases may differ significantly from that of Taq-based polymerases.

For optimal results start by accurately calculating your Tm with our Tm calculator.


Highlights


• Robust and reliable exponential amplification method
• No requirements, such as special vectors, restriction sites, or methylation status for the target plasmid
• No need to destroy the starting template in a separate step
Phusion Hot Start II High Fidelity DNA Polymerase minimizes unwanted secondary mutations
• Amplification of large plasmids up to 10 kb
• Hot start modification of the polymerase prevents amplification of non-specific products and unwanted degradation of primers prior to first cycle of PCR
• T4 DNA Ligase included in the kit; no purification steps before or after ligation
• Compatible with all strains of competent E. coli cells
For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormSolution
FormatKit
GC-Rich PCR PerformanceHigh
PolymerasePhusion Hot Start II High Fidelity DNA Polymerase
Reaction SpeedFast
Mutagenesis CapabilitySingle-Site Mutagenesis
Product LinePhusion
Product TypeSite-Directed Mutagenesis Kit
Quantity20 reactions
Fidelity (vs. Taq)52X
Hot StartYes
No. of Reactions20 Reactions
Starting MaterialDNA
Unit Size20 reactions