Thermo Scientific™

Phusion Site-Directed Mutagenesis Kit

Catalog number: F541
Thermo Scientific™

Phusion Site-Directed Mutagenesis Kit

Catalog number: F541
Catalog Number
F541
also known as F-541
Unit Size
20 reactions
Price (USD)
Price: 194.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
F541
also known as F-541
20 reactions
Price: 194.00
Your Price:
***
Product Overview
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Thermo Scientific Phusion Site-Directed Mutagenesis Kit is a versatile and efficient tool for introducing point mutations, insertions, or deletions in any type of plasmid DNA. With this kit, the entire plasmid is amplified using phosphorylated primers that introduce the desired changes. The amplified, linear PCR product, containing the desired mutation, is circularized in a 5-minute ligation reaction with T4 DNA Ligase. The resulting plasmid can be then transformed into any competent E. coli cells.

The optimal annealing temperature for Phusion DNA Polymerases may differ significantly from that of Taq-based polymerases.

For optimal results start by accurately calculating your Tm with our Tm calculator.


Highlights


• Robust and reliable exponential amplification method
• No requirements, such as special vectors, restriction sites, or methylation status for the target plasmid
• No need to destroy the starting template in a separate step
Phusion Hot Start II High Fidelity DNA Polymerase minimizes unwanted secondary mutations
• Amplification of large plasmids up to 10 kb
• Hot start modification of the polymerase prevents amplification of non-specific products and unwanted degradation of primers prior to first cycle of PCR
• T4 DNA Ligase included in the kit; no purification steps before or after ligation
• Compatible with all strains of competent E. coli cells
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Fidelity (vs. Taq)
52X
Form
Solution
Format
Kit
GC-Rich PCR Performance
High
Hot Start
Yes
No. of Reactions
20 Reactions
Polymerase
Phusion Hot Start II High Fidelity DNA Polymerase
Reaction Speed
Fast
Starting Material
DNA
Mutagenesis Capability
Single-Site Mutagenesis
Product Line
Phusion
Product Type
Site-Directed Mutagenesis Kit

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