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Phusion Blood Direct PCR Kit
Thermo Scientific Phusion Blood Direct PCR Kit is developed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR.
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| Catalog Number | No. of Reactions |
|---|---|
| F547S | 100 Reactions |
| F547L | 500 Reactions |
Catalog number F547S
Price (USD)/ 100 reactions
139.65
Online exclusive
151.00 Save 11.35 (8%)
-
No. of Reactions:
100 Reactions
Price (USD)/ 100 reactions
139.65
Online exclusive
151.00 Save 11.35 (8%)
Phusion Blood Direct PCR Kit
Catalog numberF547S
Price (USD)/ 100 reactions
139.65
Online exclusive
151.00 Save 11.35 (8%)
-
Thermo Scientific Phusion Blood Direct PCR Kit is for use in the amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR. The modified Phusion Hot Start II High-Fidelity DNA Polymerase exhibits high resistance to PCR inhibitors found in blood and retains polymerase activity even with samples containing 40% whole blood.
This DNA polymerase exhibits exceptional accuracy in proofreading and makes the Phusion Blood Direct PCR Kit one of the best choices for demanding applications, such as cloning. The Phusion Blood Direct PCR Kit is versatile and permits analysis of blood samples from various species and with different anticoagulants or storage conditions.
Features
- No need for time-consuming and expensive DNA purification steps
- Simple protocol requires minimal hands-on time
- Extremely short PCR protocol times
- Modified Phusion Hot Start II High-Fidelity DNA Polymerase delivers abundant yields of specific product
Notes
- The optimal annealing temperature for Phusion DNA Polymerases may differ significantly from that of Taq-based polymerases.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
GC-Rich PCR PerformanceHigh
PCR MethodDirect PCR
PolymerasePhusion Blood II DNA Polymerase
Reaction SpeedFast
Product TypeDirect PCR Kit
Shipping ConditionWet or Dry Ice
Concentration2 U/μL
Hot StartBuilt-In Hot Start
No. of Reactions100 Reactions
OverhangBlunt
Reaction FormatSeparate Components
Sample TypeBlood
Starting MaterialDNA
Unit Size100 reactions
Contents & Storage
• Phusion Blood II DNA Polymerase, 40 μL
• 2X Phusion Blood PCR Buffer with dNTPs and MgCl2, 1.25 mL
• Universal Control Primer Mix, 40 μL
• 50 mM EDTA, 0.5 mL
• 50 mM MgCl2, 1.5 mL
• DMSO, 0.5 mL
Store at -20°C.
• 2X Phusion Blood PCR Buffer with dNTPs and MgCl2, 1.25 mL
• Universal Control Primer Mix, 40 μL
• 50 mM EDTA, 0.5 mL
• 50 mM MgCl2, 1.5 mL
• DMSO, 0.5 mL
Store at -20°C.