Thermo Scientific™

Phusion Flash High-Fidelity PCR Master Mix

Catalog number: F548L
Thermo Scientific™

Phusion Flash High-Fidelity PCR Master Mix

Catalog number: F548L
Catalog Number
F548L
also known as F-548L
Unit Size
500 reactions
Price (USD)
Price: 391.00
Your Price:
Availability
***
Quantity
Catalog Number
F548S
also known as F-548S
Unit Size
100 reactions
Price (USD)
Price: 97.25
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
F548L
also known as F-548L
500 reactions
Price: 391.00
Your Price:
***
F548S
also known as F-548S
100 reactions
Price: 97.25
Your Price:
***
Product Overview
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Additional Information
Recommendations
Recommendations
Thermo Scientific Phusion Flash PCR Master Mix was developed to save valuable laboratory time. Ready-to-use 2X master mix preserves the fidelity and the yield in the reaction when using extremely short PCR protocols. Additionally, the user only needs to add template and primers minimizing the number of pipetting steps.

The unique master mix composition permits usage of extremely short cycling protocols with both low and high complexity DNA templates - 15 seconds per kilobase or less. The master mix utilizes Phusion Flash II DNA Polymerase, a modified proofreading DNA polymerase derived from Phusion Hot Start II High-Fidelity DNA Polymerase.

Features
• Extreme speed: extension times of 15 s/kb or less
• Hot start modification allowing “zero-time reactivation”
• Accuracy: proofreading DNA polymerase with a fidelity of 25X Taq
• High yields in reduced time

Applications

• Fast PCR
• High-fidelity PCR
• Difficult (GC-rich) templates
• Template generation for sequencing
• Multiplex PCR
• Long-range PCR
• Cloning
• Mutagenesis
• Microarray

Using Phusion DNA Polymerases
Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our Tm calculator at www.thermofisher.com/tmcalculator.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration
2X
Fidelity (vs. Taq)
25 X
Format
SuperMix or Master Mix
GC-Rich PCR Performance
High
Hot Start
Built-In Hot Start
No. of Reactions
500 Reactions
Overhang
Blunt
Polymerase
Phusion Flash II DNA Polymerase
Reaction Format
SuperMix or Master Mix
Reaction Speed
Fast
Size (Final Product)
20 kb or Less
Starting Material
DNA
Product Line
Phusion
Product Type
High-Fidelity PCR Master Mix
Quantity
500 Reactions

Contents & Storage

• 5 x 1 mL Phusion Flash Master Mix

Store at -20°C.

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