Thermo Scientific™

Phusion High-Fidelity PCR Kit

Catalog number: F553L
Thermo Scientific™

Phusion High-Fidelity PCR Kit

Catalog number: F553L
Catalog Number
F553L
also known as F-553L
Unit Size
200 reactions
Price (USD)
Price: 282.00
Your Price:
Availability
***
Quantity
Catalog Number
F553S
also known as F-553S
Unit Size
50 reactions
Price (USD)
Price: 84.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
F553L
also known as F-553L
200 reactions
Price: 282.00
Your Price:
***
F553S
also known as F-553S
50 reactions
Price: 84.00
Your Price:
***
Product Overview
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Additional Information
Recommendations
Recommendations
Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq, and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity. Phusion DNA Polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerase.

Highlights

• High fidelity (52X Taq)
• Fast PCR due to short extension times (15-30 s/kb)
• Robust performance, minimal optimization needed
• High yields of PCR products with minimal enzyme amounts
• Available as a Green buffer format for direct loading of PCR products on gels (F-534S or F-534L)

Applications

• High-fidelity PCR
• Cloning
• Template generation for sequencing
• Amplification of difficult (GC-rich) templates
• Long-range PCR (up to 20 kb)
• Mutagenesis
• High throughput PCR
• Microarray
Thermo Scientific Phusion High-Fidelity PCR Kit contains all the necessary reagents for PCR including control lambda DNA template and primers for 1.3 kb and 10 kb amplicons. The DNA template amount is sufficient for performing 20 control reactions in 50 µL volume or 50 control reactions in 20 µL volume.

Using Phusion DNA Polymerases
Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our Tm calculator at www.thermofisher.com/tmcalculator.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Application)
PCR Amplification
Fidelity (vs. Taq)
52 X
Format
Kit
GC-Rich PCR Performance
High
Hot Start
No
No. of Reactions
200 Reactions
Overhang
Blunt
Polymerase
Phusion High-Fidelity DNA Polymerase
Reaction Format
Separate Components
Reaction Speed
Fast
Size (Final Product)
20 kb
Starting Material
DNA
Includes
Phusion DNA Polymerase, 5x Phusion HF Buffer, 5x Phusion GC Buffer, dNTP Mix, MgCl2 solution, control lambda DNA, 1.3kb control primers, 10kb control primers, DNA size standard, DMS
Product Line
Phusion
Product Type
High-Fidelity PCR Kit
Quantity
200 x 50 μL Reactions (or 500 x 20 μL Reactions)

Contents & Storage

Includes:
• Phusion DNA Polymerase (2 U/µL)
• 5X Phusion GC Buffer
• 5X Phusion HF Buffer
• dNTP mix (10 mM each)
• MgCl2 solution (50 mM)
• Control template (λ DNA)
• 1.3 kb primers (4 µM each)
• 10 kb primers (4 µM each)
• DNA size standard
• DMSO

Store at -20°C.

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