Thermo Scientific™

Phusion U Multiplex PCR Master Mix

Catalog number: F562L
Thermo Scientific™

Phusion U Multiplex PCR Master Mix

Catalog number: F562L
Catalog Number
F562L
also known as F-562L
Unit Size
500 reactions
Price (USD)
Price: 1,058.00
Your Price:
Availability
***
Quantity
Catalog Number
F562S
also known as F-562S
Unit Size
100 reactions
Price (USD)
Price: 266.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
F562L
also known as F-562L
500 reactions
Price: 1,058.00
Your Price:
***
F562S
also known as F-562S
100 reactions
Price: 266.00
Your Price:
***
Product Overview
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Thermo Scientific Phusion U Multiplex PCR Master Mix is a ready-to-use, 2X end-point PCR master mix designed for simultaneous amplification of multiple targets in a single tube. Over 20 primer pairs may be combined into a single reaction for highly specific and efficient multiplexing over a broad range of primer and template concentrations.

The Phusion U Multiplex PCR Master Mix contains a proprietary buffer with balanced concentrations of all PCR components eliminating the need for tedious optimization. The Phusion U Green Multiplex PCR Master Mix (F-564S or F-564L) further simplifies the workflow - it includes a density reagent and two electrophoresis tracking dyes for direct loading of PCR products on gels.

Highlights

• Specific and efficient multiplex PCR of over 20 targets up to 2.5 kb
• Excellent results with templates of suboptimal purity due to inhibitor tolerance
• Fast cycling and direct loading of PCR products on gels with Green format (F-564S and F-564L)

Applications

• Genotyping
• Pathogen detection
• Food testing
• Analysis of genetically modified organisms
• Amplification of microsatellites

The master mix is based on Phusion U Hot Start DNA Polymerase, a high performance enzyme developed using fusion technology. Similarly to other enzymes from Phusion family, Phusion U DNA Polymerase incorporates more nucleotides per binding event than any other DNA polymerase. This allows achieving high yields of PCR products with shorter extention times and consequently reduced total protocol times. Significantly enhanced processivity also enables successful multiplex PCR on difficult targets such as GC-rich templates or templates of suboptimal purity. Being highly tolerant of many PCR inhibitors, Phusion U Multiplex PCR Master Mix can even be used with unpurified samples such as blood or serum.

Specificity of multiplex PCR is increased by Affibody-mediated hot start mechanism. Reversibly bound Affibody ligand inhibits the polymerase at ambient temperatures preventing amplification of nonspecific products and formation of primer-dimers.

Using Phusion DNA Polymerases
Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our Tm calculator at www.thermofisher.com/tmcalculator.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration
2X
Fidelity (vs. Taq)
25X
Format
Tube
GC-Rich PCR Performance
Medium
Hot Start
Built-In Hot Start
No. of Reactions
500
Overhang
Blunt
Polymerase
Phusion U Hot Start DNA Polymerase
Reaction Format
SuperMix or Master Mix
Reaction Speed
Fast
Size (Final Product)
2.5 kb or Less
Starting Material
DNA
Product Line
Phusion
Product Type
Multiplex PCR Master Mix
Quantity
500 x 50 μL Reactions

Contents & Storage

Phusion U Multiplex PCR Master Mix includes:
• Phusion U Hot Start DNA Polymerase
• 2X reaction buffer
• 3 mM MgCl2
• 400 µM of each dATP, dTTP, dCTP and dGTP
The master mix is supplied with nuclease-free water.
Store at -20°C.

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