Phusion U Multiplex PCR Master Mix, 100 reactions - FAQs

For direct multiplex PCR without prior DNA purification, add up to 4 µL of whole blood to 50 µL final reaction volume.

Yes, Phusion U Multiplex PCR Master Mix can be stored at +4 degrees C for up to 3 months with no effect on performance.

Phusion U Multiplex PCR Master Mix is designed for simultaneous amplification of targets up to 2.5 kb.

We have demonstrated successful simultaneous amplification of 24 DNA fragments.

Thermo Scientific Phusion U Multiplex PCR Master Mixes (Cat. No. F562S, F564S, F562L, F564L) are ready-to-use, 2X end-point PCR master mixes designed for simultaneous amplification of multiple targets in a single tube. Over 20 primer pairs may be combined into a single reaction for highly specific and efficient multiplexing over a broad range of primer and template concentrations. Phusion U Multiplex PCR Master Mixes contains a proprietary buffer with balanced concentrations of all PCR components eliminating the need for tedious optimization.

The Green format of the Phusion U Multiplex PCR Master Mixes (Cat. No. F564S, F564L) further simplifies the workflow: it includes a density reagent and two electrophoresis tracking dyes for direct loading of PCR products on gels.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

Yes, Phusion U Multiplex PCR Master Mix contains Phusion U Hot Start DNA Polymerase which is a novel engineered high-fidelity enzyme developed using fusion technology. Due to a proprietary mutation in the so-called dUTP binding pocket of Phusion, Phusion U overcomes an important limitation of proofreading enzymes.