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View additional product information for Phusion U Multiplex PCR Master Mixes - FAQs (F562L, F564S, F562S, F564L)
7 product FAQs found
For direct multiplex PCR without prior DNA purification, add up to 4 µL of whole blood to 50 µL final reaction volume.
Yes, Phusion U Multiplex PCR Master Mix can be stored at +4 degrees C for up to 3 months with no effect on performance.
Phusion U Multiplex PCR Master Mix is designed for simultaneous amplification of targets up to 2.5 kb.
We have demonstrated successful simultaneous amplification of 24 DNA fragments.
Thermo Scientific Phusion U Multiplex PCR Master Mixes (Cat. No. F562S, F564S, F562L, F564L) are ready-to-use, 2X end-point PCR master mixes designed for simultaneous amplification of multiple targets in a single tube. Over 20 primer pairs may be combined into a single reaction for highly specific and efficient multiplexing over a broad range of primer and template concentrations. Phusion U Multiplex PCR Master Mixes contains a proprietary buffer with balanced concentrations of all PCR components eliminating the need for tedious optimization.
The Green format of the Phusion U Multiplex PCR Master Mixes (Cat. No. F564S, F564L) further simplifies the workflow: it includes a density reagent and two electrophoresis tracking dyes for direct loading of PCR products on gels.
Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.
Yes. The dyes do not interfere with downstream applications such as DNA sequencing, ligation, and restriction digestion.
Phusion Green and Phire Green buffers contain two dyes for monitoring electrophoresis progress. The blue dye migrates with 3-5 kb DNA fragments and the yellow dye migrates faster than 10 bp DNA fragments in 1% agarose gel. The dyes have excitation peaks at 424 nm and 615 nm, respectively.