Viewing dynamic assembly of molecular complexes by multi-wavelength single-molecule fluorescence.
AuthorsFriedman LJ, Chung J, Gelles J
JournalBiophys J
PubMed ID16698779
'Complexes of macromolecules that transiently self-assemble, perform a particular function, and then dissociate are a recurring theme in biology. Such systems often have a large number of possible assembly/disassembly intermediates and complex, highly branched reaction pathways. Measuring the single-step kinetic parameters in these reactions would help to identify the functionally ... More
Ultrathin self-assembled fibrin sheets.
AuthorsO'Brien ET, Falvo MR, Millard D, Eastwood B, Taylor RM, Superfine R,
JournalProc Natl Acad Sci U S A
PubMed ID19052234
'Fibrin polymerizes into the fibrous network that is the major structural component of blood clots and thrombi. We demonstrate that fibrin from three different species can also spontaneously polymerize into extensive, molecularly thin, 2D sheets. Sheet assembly occurs in physiologic buffers on both hydrophobic and hydrophilic surfaces, but is routinely ... More
A murine scavenger receptor MARCO recognizes polystyrene nanoparticles.
AuthorsKanno S, Furuyama A, Hirano S,
JournalToxicol Sci
PubMed ID17361018
'Recent toxicological studies indicate that nanoparticles or ultrafine particles (< 100 nm) are more toxic than fine particles (< 2 microm) because of their greater surface area. It is well known that alveolar macrophages play an important role in the first defense against various environmental particles and microorganisms. This is ... More
The impact of different nanoparticle surface chemistry and size on uptake and toxicity in a murine macrophage cell line.
AuthorsClift MJ, Rothen-Rutishauser B, Brown DM, Duffin R, Donaldson K, Proudfoot L, Guy K, Stone V,
JournalToxicol Appl Pharmacol
PubMed ID18708083
This study investigated the uptake, kinetics and cellular distribution of different surface coated quantum dots (QDs) before relating this to their toxicity. J774.A1 cells were treated with organic, COOH and NH2 (PEG) surface coated QDs (40 nM). Model 20 nm and 200 nm COOH-modified coated polystyrene beads (PBs) were also ... More
Light transmission technique for the evaluation of colloidal transport and dynamics in porous media.
AuthorsWeisbrod N, Niemet MR, Selker JS
JournalEnviron Sci Technol
PubMed ID12953883
Colloidal transport in porous media has been typically studied in column experiments from which data analysis was limited to the evaluation of effluent breakthrough curves and/or destructive sampling at the end of the experiments. The internal processes occur within a "black box", where direct observation is not possible and therefore ... More
Primitive, crustacean-like state of blood-brain barrier in the eye of the apterygote insect Petrobius (Archaeognatha) determined from uptake of fluorescent tracers.
AuthorsShaw SR, Varney LP
JournalJ Neurobiol
PubMed ID10590170
Compound eyes of insects in 16 orders were tested for the presence of a blood-retina barrier (BRB) by injecting the hemolymph with Procion yellow, which was excluded from the eye in all Neoptera but not in two apterygotes. A primitive apterygote (Petrobius, Machilidae) was investigated further. Epifluorescence observations with small ... More
A critical role for calponin 2 in vascular development.
AuthorsTang J, Hu G, Hanai J, Yadlapalli G, Lin Y, Zhang B, Galloway J, Bahary N, Sinha S, Thisse B, Thisse C, Jin JP, Zon LI, Sukhatme VP
JournalJ Biol Chem
PubMed ID16317011
Calponin 2 (h2 calponin, CNN2) is an actin-binding protein implicated in cytoskeletal organization. We have found that the expression of calponin 2 is relatively restricted to vasculature from 16 to 30 h post-fertilization during zebrafish (Danio rerio) development. Forty-eight hours after injecting antisense morpholino oligos against calponin 2 into embryos ... More