'Molecules drift along temperature gradients, an effect called thermophoresis, the Soret effect, or thermodiffusion. In liquids, its theoretical foundation is the subject of a long-standing debate. By using an all-optical microfluidic fluorescence method, we present experimental results for DNA and polystyrene beads over a large range of particle sizes, salt ... More
A murine scavenger receptor MARCO recognizes polystyrene nanoparticles.
AuthorsKanno S, Furuyama A, Hirano S,
JournalToxicol Sci
PubMed ID17361018
'Recent toxicological studies indicate that nanoparticles or ultrafine particles (< 100 nm) are more toxic than fine particles (< 2 microm) because of their greater surface area. It is well known that alveolar macrophages play an important role in the first defense against various environmental particles and microorganisms. This is ... More
Oligomeric tubulin in large transporting complex is transported via kinesin in squid giant axons.
AuthorsTerada S, Kinjo M, Hirokawa N
JournalCell
PubMed ID11051554
Slow axonal transport depends on an active mechanism that conveys cytosolic proteins. To investigate its molecular mechanism, we now constructed an in vitro experimental system for observation of tubulin transport, using squid giant axons. After injecting fluorescence-labeled tubulin into the axons, we monitored the movement of fluorescence by confocal laser ... More
A nanoparticle-based model delivery system to guide the rational design of gene delivery to the liver. 1. Synthesis and characterization.
AuthorsPopielarski SR, Pun SH, Davis ME,
JournalBioconjug Chem
PubMed ID16173781
Nonviral gene delivery systems are amenable to forming colloidal particles with a wide range of physicochemical properties that include size, surface charge, and density and type of ligand presented. However, it is not known how to best design these particles without having a set of physicochemical design constraints that have ... More
Optical detection and charge-state analysis of MALDI-generated particles with molecular masses larger than 5 MDa.
AuthorsCai Y, Peng WP, Kuo SJ, Sabu S, Han CC, Chang HC
JournalAnal Chem
PubMed ID12236352
Charged polystyrene nanoparticles are generated by matrix-assisted laser desorption/ionization (MALDI) and detected by laser-induced fluorescence (LIF) in a quadrupole ion trap. Employing the LIF technique, observations of individual fluorescent nanospheres (27 nm in diameter and containing 180 fluorescein dye equivalents) have been achieved with an average signal-to-noise ratio of approximately ... More
Detection of changes occurring during recovery from the dauer stage in Heterorhabditis bacteriophora.
AuthorsDolan KM, Jones JT, Burnell AM
JournalParasitology
PubMed ID12166523
Nematodes of the genus Heterorhabditis are insect parasites that are widely used as biological control agents. When conditions are unfavourable for reproduction in H. bacteriophora, a long-lived, non-feeding, survival and dispersal stage, the dauer juvenile (DJ), is formed. This DJ stage is also adapted for host finding and infection. When ... More
Spatially correlated fluorescence/AFM of individual nanosized particles and biomolecules.
AuthorsKolodny LA, Willard DM, Carillo LL, Nelson MW, Van Orden A
JournalAnal Chem
PubMed ID11354476
Individual fluorescent polystyrene nanospheres (<10-100-nm diameter) and individual fluorescently labeled DNA molecules were dispersed on mica and analyzed using time-resolved fluorescence spectroscopy and atomic force microscopy (AFM). Spatial correlation of the fluorescence and AFM measurements was accomplished by (1) positioning a single fluorescent particle into the near diffraction-limited confocal excitation ... More