Nano-sized fluorescent particles as new tracers for sentinel node detection: experimental model for decision of appropriate size and wavelength.
AuthorsNakajima M, Takeda M, Kobayashi M, Suzuki S, Ohuchi N
JournalCancer Sci
PubMed ID15958058
'The concepts of made-to-order and low-invasiveness medicines are becoming widely accepted. A treatment for cancer, with minimum invasive surgery and without lymph nodes dissection based on sentinel lymph node (SN) navigation surgery, would adhere to these concepts. Dyes and/or radioisotopes are employed for SN detection in standard methods, however, each ... More
Why molecules move along a temperature gradient.
AuthorsDuhr S, Braun D
JournalProc Natl Acad Sci U S A
PubMed ID17164337
'Molecules drift along temperature gradients, an effect called thermophoresis, the Soret effect, or thermodiffusion. In liquids, its theoretical foundation is the subject of a long-standing debate. By using an all-optical microfluidic fluorescence method, we present experimental results for DNA and polystyrene beads over a large range of particle sizes, salt ... More
AuthorsPerrin-Cocon L, Coutant F, Agaugué S, Deforges S, André P, Lotteau V
JournalJ Immunol
PubMed ID11564795
'Proinflammatory oxidized phospholipids are generated during oxidative modification of low-density lipoproteins (LDL). The production of these proinflammatory oxidized phospholipids is controlled by secreted enzymes that circulate as proteins complexed with LDL and high-density lipoprotein. During the acute phase response to tissue injury, profound changes occur in lipoprotein enzymatic composition that ... More
I5S: wide-field light microscopy with 100-nm-scale resolution in three dimensions.
AuthorsShao L, Isaac B, Uzawa S, Agard DA, Sedat JW, Gustafsson MG,
JournalBiophys J
PubMed ID18326649
A new type of wide-field fluorescence microscopy is described, which produces 100-nm-scale spatial resolution in all three dimensions, by using structured illumination in a microscope that has two opposing objective lenses. Illumination light is split by a grating and a beam splitter into six mutually coherent beams, three of which ... More
Determination of absolute protein numbers in single synapses by a GFP-based calibration technique.
AuthorsSugiyama Y, Kawabata I, Sobue K, Okabe S,
JournalNat Methods
PubMed ID16118638
To build a quantitative model of molecular organization of neurons, it is essential to have information about the number of protein molecules at individual synapses. Here we developed a method to estimate absolute numbers of individual proteins at actual excitatory synapses by calibrating the fluorescence intensity of microspheres with single ... More
Visualization of synaptic vesicle movement in intact synaptic boutons using fluorescence fluctuation spectroscopy.
AuthorsJordan R, Lemke EA, Klingauf J
JournalBiophys J
PubMed ID15980175
Not much is known about the mobility of synaptic vesicles inside small synapses of the central nervous system, reflecting a lack of methods for visualizing these dynamics. We adapted confocal spot detection with fluctuation analysis to monitor the mobility of fluorescently labeled synaptic vesicles inside individual boutons of cultured hippocampal ... More
Preparation of DNA-modified nanoparticles and preliminary study for colorimetric SNP analysis using their selective aggregations.
AuthorsIhara T, Tanaka S, Chikaura Y, Jyo A
JournalNucleic Acids Res
PubMed ID15254253
DNA-modified nanospheres were prepared by anchoring amino-terminated oligodeoxynucleotides (ODNs) with carboxylates onto a colored polystyrene sphere surface through amido bonds. About 220 ODN molecules were immobilized onto a nanosphere 40 nm in diameter. Preliminary studies using the microspheres with 1 microm diameter reveal that the specificity of hybridization was retained ... More
Spatially correlated fluorescence/AFM of individual nanosized particles and biomolecules.
AuthorsKolodny LA, Willard DM, Carillo LL, Nelson MW, Van Orden A
JournalAnal Chem
PubMed ID11354476
Individual fluorescent polystyrene nanospheres (<10-100-nm diameter) and individual fluorescently labeled DNA molecules were dispersed on mica and analyzed using time-resolved fluorescence spectroscopy and atomic force microscopy (AFM). Spatial correlation of the fluorescence and AFM measurements was accomplished by (1) positioning a single fluorescent particle into the near diffraction-limited confocal excitation ... More