Syntaxin 7 is localized to late endosome compartments, associates with Vamp 8, and Is required for late endosome-lysosome fusion.
AuthorsMullock BM, Smith CW, Ihrke G, Bright NA, Lindsay M, Parkinson EJ, Brooks DA, Parton RG, James DE, Luzio JP, Piper RC
JournalMol Biol Cell
PubMed ID10982406
'Protein traffic from the cell surface or the trans-Golgi network reaches the lysosome via a series of endosomal compartments. One of the last steps in the endocytic pathway is the fusion of late endosomes with lysosomes. This process has been reconstituted in vitro and has been shown to require NSF, ... More
Estimated functional diameter of alveolar septal microvessels in zone 1.
AuthorsConhaim RL, Rodenkirch LA
JournalAm J Physiol
PubMed ID8853334
'To estimate the functional diameter of alveolar septal microvessels in zone 1, we perfused isolated rat lungs with fluorescent latex particles of specific diameters (0.24, 0.49, 1.05, or 4.0 microns) at pulmonary artery pressures (Ppulmart) that were either 5 or 10 cmH2O less than the air inflation pressure (Pinflat, 25 ... More
A murine scavenger receptor MARCO recognizes polystyrene nanoparticles.
AuthorsKanno S, Furuyama A, Hirano S,
JournalToxicol Sci
PubMed ID17361018
'Recent toxicological studies indicate that nanoparticles or ultrafine particles (< 100 nm) are more toxic than fine particles (< 2 microm) because of their greater surface area. It is well known that alveolar macrophages play an important role in the first defense against various environmental particles and microorganisms. This is ... More
Ehrlichia chaffeensis infections in Drosophila melanogaster.
AuthorsLuce-Fedrow A, Von Ohlen T, Chapes SK,
JournalInfect Immun
PubMed ID19687202
Ehrlichia chaffeensis is an obligate, intracellular bacterium, transmitted by the tick Amblyomma americanum, and is the causative agent of human monocytic ehrlichiosis infections. We previously demonstrated that E. chaffeensis is capable of growing in Drosophila S2 cells. Therefore, we tested the hypothesis that E. chaffeensis can infect adult Drosophila melanogaster. ... More
Enhanced viscoelasticity of human cystic fibrotic sputum correlates with increasing microheterogeneity in particle transport.
AuthorsDawson M, Wirtz D, Hanes J,
JournalJ Biol Chem
PubMed ID13679362
Current biochemical characterizations of cystic fibrosis (CF) sputum do not address the high degree of microheterogeneity in the rheological properties of the mucosal matrix and only provide bulk-average particle diffusion coefficients. The viscoelasticity of CF sputum greatly reduces the diffusion rates of colloidal particles, limiting the effectiveness of gene delivery ... More
The impact of different nanoparticle surface chemistry and size on uptake and toxicity in a murine macrophage cell line.
AuthorsClift MJ, Rothen-Rutishauser B, Brown DM, Duffin R, Donaldson K, Proudfoot L, Guy K, Stone V,
JournalToxicol Appl Pharmacol
PubMed ID18708083
This study investigated the uptake, kinetics and cellular distribution of different surface coated quantum dots (QDs) before relating this to their toxicity. J774.A1 cells were treated with organic, COOH and NH2 (PEG) surface coated QDs (40 nM). Model 20 nm and 200 nm COOH-modified coated polystyrene beads (PBs) were also ... More
Tracking single proteins within cells.
AuthorsGoulian M, Simon SM
JournalBiophys J
PubMed ID11023923
We present experiments in which single proteins were imaged and tracked within mammalian cells. Single proteins of R-phycoerythrin (RPE) were imaged by epifluorescence microscopy in the nucleoplasm and cytoplasm at 71 frames/s. We acquired two-dimensional trajectories of proteins (corresponding to the projection of three-dimensional trajectories onto the plane of focus) ... More
Light transmission technique for the evaluation of colloidal transport and dynamics in porous media.
AuthorsWeisbrod N, Niemet MR, Selker JS
JournalEnviron Sci Technol
PubMed ID12953883
Colloidal transport in porous media has been typically studied in column experiments from which data analysis was limited to the evaluation of effluent breakthrough curves and/or destructive sampling at the end of the experiments. The internal processes occur within a "black box", where direct observation is not possible and therefore ... More
Primitive, crustacean-like state of blood-brain barrier in the eye of the apterygote insect Petrobius (Archaeognatha) determined from uptake of fluorescent tracers.
AuthorsShaw SR, Varney LP
JournalJ Neurobiol
PubMed ID10590170
Compound eyes of insects in 16 orders were tested for the presence of a blood-retina barrier (BRB) by injecting the hemolymph with Procion yellow, which was excluded from the eye in all Neoptera but not in two apterygotes. A primitive apterygote (Petrobius, Machilidae) was investigated further. Epifluorescence observations with small ... More