Fractal nature of regional ventilation distribution.
AuthorsAltemeier WA, McKinney S, Glenny RW
JournalJ Appl Physiol
PubMed ID10797111
'High-resolution measurements of pulmonary perfusion reveal substantial spatial heterogeneity that is fractally distributed. This observation led to the hypothesis that the vascular tree is the principal determinant of regional blood flow. Recent studies using aerosol deposition show similar ventilation heterogeneity that is closely correlated with perfusion. We hypothesize that ventilation ... More
Perfluorohexane vapor has only minor effects on spatial pulmonary blood flow distribution in isolated rabbit lungs.
AuthorsHübler M, Heller AR, Bleyl JU, de Abreu MG, Kroll T, Rössel T, Koch T
JournalAnesth Analg
PubMed ID15781532
'We tested the hypothesis that administration of perfluorohexane (PFH) vapor does not significantly affect the relative pulmonary blood flow (Qrel) distribution in isolated rabbit lungs. Fourteen isolated rabbit lungs were perfused with a Krebs-Henseleit buffer solution (flow 150 mL/min). Pulmonary afterload was set to 3 mm Hg. The lungs were ... More
A modified fluorescent microsphere-based approach for determining resting and hyperemic blood flows in individual murine skeletal muscles.
AuthorsCardinal TR, Hoying JB,
JournalVascul Pharmacol
PubMed ID17500044
'The goal of this study was to develop a modified fluorescent microsphere-based approach for measuring resting and hyperemic blood flows in individual mouse skeletal muscles. Absolute resting blood flow in the left gracilis posterior was 1.04+/-0.12 ml x min(-1).g(-1), while functional hyperemia following muscle activity was 5.94+/-1.33 ml x min(-1) ... More
Validation of fluorescent-labeled microspheres for measurement of regional organ perfusion.
AuthorsGlenny RW, Bernard S, Brinkley M
JournalJ Appl Physiol
PubMed ID8335595
'Estimations of dog lung, pig heart, and pig kidney regional perfusion by use of fluorescent-labeled microspheres were compared with measurements obtained with standard radiolabeled microspheres. Pairs of radio- and fluorescent-labeled microspheres (15 microns diam, 6 colors) were injected into a central vein of a supine anesthetized dog and the left ... More
Development of a fluorescent microsphere technique for rapid histological determination of cerebral blood flow.
'The purpose of this study was to develop a more efficient fluorescent microsphere method to facilitate the rapid use of the histological technique and to enable its use in large tissue regions. Using fluorescent plate/slide imaging technology and automated detection and analysis software, we were able to rapidly image, detect, ... More
Measuring bone blood supply in mice using fluorescent microspheres.
AuthorsSerrat MA,
JournalNat Protoc
PubMed ID19893510
Fluorescent microspheres are commonly used to assess bone blood supply in large animals, but the technique is not widely used in smaller mammals, as traditional methods such as reference blood sampling, ventilation and catheterization are not easily applied. This protocol describes a viable alternative for measuring bone and organ perfusion ... More
Development of a novel fluorescent microsphere technique to combine serial cerebral blood flow measurements with histology in the rat.
AuthorsDe Visscher G, Haseldonckx M, Flameng W, Borgers M, Reneman RS, van Rossem K
JournalJ Neurosci Methods
PubMed ID12573474
The aim of the study was to evaluate the microsphere technique for the quantitative assessment of regional cerebral blood flow (rCBF) at different time points in the same animal. Yellow-green and red fluorescent microspheres with a diameter of 15 microm were injected into the rat at two different time points ... More
Fluorescent microspheres to measure organ perfusion: validation of a simplified sample processing technique.
A disadvantage of nonradioactive microsphere techniques is that the processing of samples is time-consuming and complex. We developed and validated a simplified processing method for the fluorescent microsphere (FM) technique. In seven anesthetized dogs with coronary artery stenosis up to six different FM and five different radioactivity labeled microspheres (RM) ... More