Invitrogen™

Cell Lysis Buffer

Catalog number: FNN0011
Invitrogen™

Cell Lysis Buffer

Catalog number: FNN0011
Catalog Number
FNN0011
Unit Size
100 ml
Price (USD)
Price: 162.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
FNN0011100 ml
Price: 162.00
Your Price:
-
Product Overview
Figures
Videos
Recommendations
Recommendations
Documents
FAQ
Citations & References
Additional Information
Recommendations
Cell Lysis Buffer is a ready-to-use lysis buffer for use in ELISA and western blotting applications for total protein extraction from mammalian cells. The buffer uses detergent-based lysis, eliminating the need for mechanical cell disruption, providing a milder and easier alternative when isolating proteins from cell cultures. The formulation helps retain the protein structure and function needed for enzyme assays or immunoassays.

Buffer formulation: 10 mM Tris, pH 7.4, 100 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1 mM NaF, 20 mM Na4P2O7
2 mM Na3VO4, 1% Triton X-100, 10% glycerol, 0.1% SDS, 0.5% deoxycholate

This cell extraction buffer does not contain protease inhibitors and should be supplemented with 1 mM PMSF and protease inhibitor cocktail just prior to use.

View all available ELISA lysis buffers and reagents.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type
Cell Extraction Buffer
For Use With (Application)
Western Blot, Sample preparation for ELISA
Shipping Condition
Dry Ice

Contents & Storage

Store in freezer (-5 to -30°C).