Invitrogen™

Cell Lysis Buffer

Catalog number:  FNN0011

Related applications:

Protein Assays and Analysis

| Protein Purification & Isolation

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Description

Cell Lysis Buffer is a ready-to-use lysis buffer for use in ELISA and western blotting applications for total protein extraction from mammalian cells. The buffer uses detergent-based lysis, eliminating the need for mechanical cell disruption, providing a milder and easier alternative when isolating proteins from cell cultures. The formulation helps retain the protein structure and function needed for enzyme assays or immunoassays.

Buffer formulation: 10 mM Tris, pH 7.4, 100 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1 mM NaF, 20 mM Na4P2O7
2 mM Na3VO4, 1% Triton X-100, 10% glycerol, 0.1% SDS, 0.5% deoxycholate

This cell extraction buffer does not contain protease inhibitors and should be supplemented with 1 mM PMSF and protease inhibitor cocktail just prior to use.

View all available ELISA lysis buffers and reagents.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type: Cell Extraction Buffer
For Use With (Application): Western Blot, Sample preparation for ELISA
Shipping Condition: Dry Ice

Contents & storage

Store in freezer (-5 to -30°C).