Tissue Extraction Reagent I
Tissue Extraction Reagent I
Citas y referencias (1)
Invitrogen™

Tissue Extraction Reagent I

El reactivo de extracción de tejidos I es un tampón de lisis a base de Tris listo para su usoMás información
Have Questions?
Número de catálogoCantidad
FNN0071100 mL
Número de catálogo FNN0071
Precio (MXN)
-
Cantidad:
100 mL
El reactivo de extracción de tejidos I es un tampón de lisis a base de Tris listo para su uso para la extracción total de proteínas de muestras de tejidos mediante homogeneización. Los lisados celulares preparados con el reactivo I de extracción de tejidos son compatibles con inmunoensayos como ELISA, Western blot y Luminex.

Formulación del tampón: 50 mm de Tris, pH 7,4, 250 mm de NaCl, 5 mm de EDTA, 2 mm de Na3VO4, 1 mm de Naf, 20 mm de Na4P2O7, 0,02 % de NaN3, detergente

Este tampón de lisis celular no contiene inhibidores de proteasa y debe suplementarse con 1 mm de PMSF y cóctel inhibidor de proteasa justo antes de su uso.

El reactivo de extracción de tejidos I puede complementarse con sales, agentes reductores o agentes quelantes según sea necesario.

Ver todos los reactivos y tampones de lisis ELISA disponibles.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Para utilizar con (aplicación)Ensayo Luminex, Western blot, preparación de muestras para ELISA
Tipo de productoReactivo de extracción de tejidos
Cantidad100 mL
Condiciones de envíoHielo seco
Tipo de etiquetaEtiquetado con enzimas
Unit SizeEach
Contenido y almacenamiento
Tampón. Estable durante 2 o 3 semanas a 2-8 °C o hasta 1 año cuando se distribuye en alícuotas de trabajo y se almacena a ≤20 °C.

Preguntas frecuentes

What extraction reagents are recommended for efficient mouse tissue analysis?

We have 5 different cell and tissue extraction buffers suitable for preparing mouse cell and tissue extracts. These buffers can be used to extract cells and tissues from many other species as well. The exact compositions of all of our buffers are proprietary, but they are similar to those described by many researchers.

Four of these buffers can be used to prepare extracts which can be analyzed with our ELISA and Luminex kits and by Western blotting. Our Cell Extraction Buffer (FNN0011) contains extra phosphatase inhibitors and resembles the RIPA formulation that many people use. Our Tissue Extraction Reagents I (FNN0071) and II (FNN0081) contain different concentrations of NaCl and different surfactants, but are otherwise similar to each other. For those who prefer using an extraction buffer containing the detergent NP-40, we have our NP-40 Lysis Buffer (FNN0021). Finally, we sell a Denaturing Cell Extraction buffer (FNN0091) which contains 3 detergents and a chaotropic agent. Extracts prepared with FNN0091 can be analyzed with our ELISA kits and by Western blotting only. These buffers do not contain protease inhibitors, which the investigator should add right before use.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is the difference in application between Tissue Extraction Reagent I (Cat. No. FNN0071) and Tissue Extraction Reagent II (Cat. No. FNN0081)?

The main difference is that Tissue Extraction Reagent II is more stringent than Tissue Extraction Reagent I. Additionally, please note that Tissue Extraction Reagent I contains EDTA which may not be compatible with some downstream applications.

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.

Is Tissue Extraction Reagent I (Cat. No. FNN0071) compatible with the BCA Protein Assay Kit (Cat. No. 23227, 23225)?

Sorry, we have not tested the compatibility of Tissue Extraction Reagent I with our protein assays.

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.

During my ProcartaPlex assay data analysis, I am getting a warning message that there is high bead aggregation. What should I do?

Here are possible causes and solutions for this issue:

- Check the protocol settings (make sure you select the correct DD settings).
- Check the level of sheath fluid and empty the waste.
- Before acquiring the plate, run calibration and verification beads on the Luminex instrument.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

During my ProcartaPlex assay data analysis, the beads fall below or to the lower left of their bead region on the bead map. Why is this?

Here are possible causes and solutions for this issue:

This usually indicates that the beads have been photobleached. This problem can also be caused by exposing the beads to organic solvents. Unfortunately, the assay will have to be repeated because the beads cannot be restored. The beads must be protected from light and organic solvents.
Alternatively, the instrument may be off in its measurements or you may have a calibration issue. Call the manufacturer for a service appointment.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

View all Tissue Extraction Reagent I FAQs

Citations & References (1)

Citations & References
Abstract
Novel method of monitoring trace cytokines and activated STAT molecules in the paws of arthritic mice using multiplex bead technology.
Authors:Lu LD, Stump KL, Seavey MM
Journal:BMC Immunol
PubMed ID:21073728
The use of mouse models to study human disease provides useful data that can provide support for research projects or an existing drug discovery program. How well a model recapitulates the human condition and the ease and reproducibility of data collected will determine how much confidence a scientist can place ... More