Invitrogen™

Tissue Extraction Reagent I

Catalog number:  FNN0071

Related applications:

Protein Assays and Analysis

| Protein Purification & Isolation

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Description

Tissue Extraction Reagent I is a ready-to-use Tris-based lysis buffer for total protein extraction from tissue samples by homogenization. Cell lysates prepared with Tissue Extraction Reagent I are compatible with immunoassays such as ELISA, western blot, and Luminex assay.

Buffer formulation: 50 mM Tris, pH 7.4, 250 mM NaCl, 5 mM EDTA, 2 mM Na3VO4, 1 mM NaF, 20 mM Na4P2O7, 0.02% NaN3, detergent

This cell lysis buffer does not contain protease inhibitors and should be supplemented with 1 mM PMSF and protease inhibitor cocktail just prior to use.

Tissue Extraction Reagent I may be supplemented with salts, reducing agents, or chelating agents as needed.

View all available ELISA lysis buffers and reagents.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type: Tissue Extraction Reagent
For Use With (Application): Luminex Assay, Western Blot, Sample preparation for ELISA
Label Type: Enzyme Labeled
Shipping Condition: Dry Ice

Contents & storage

Buffer. Stable for 2-3 weeks at 2-8°C or for up to 1 year when apportioned into working aliquots and stored at ≤20°C.