Guanosine 5'-O-(3-Thiotriphosphate), BODIPY® FL Thioester, Sodium Salt (BODIPY® FL GTP-γ-S, Thioester)

Catalog number: G22183

Molecular Probes™  Related applications: Protein Assays and Analysis | Target & Lead Identification & Validation

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Description

BODIPY FL GTP-γ-S binds to GTP-binding proteins. To create this fluorescent nucleotide, the BODIPY FL fluorophore was linked through the γ-thiol of GTP-γ-S. The fluorescence of BODIPY FL GTP-γ-S is quenched ~90% relative to that of the free dye, but may be recovered on binding to G-proteins. In addition to its potential use for binding studies, the BODIPY FL GTP-γ-S thioether is an important new substrate for Fhit, a member of the histidine triad superfamily of nucleotide-binding proteins.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Label or Dye: BODIPY® FL
Product Size: 50 µL
Concentration: 5 mM
Labeling Method: Direct Labeling, Chemical Labeling
Shipping Condition: Wet Ice

Contents & storage

Store in freezer (-5 to -30°C) and protect from light.

Documents

Manuals & protocols