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Zitierungen und Referenzen (15)
Invitrogen™
Glutathione Ethyl Ester, Biotin Amide (BioGEE) (Glutathiolation Detection Reagent) - Special Packaging
Biotinyliertes Glutathion-Ethylester (BioGEE) ist ein membrangängiges, biotinyliertes Glutathion-Analogon zum Nachweis von Glutathiolation. Unter oxidativem Stress können Zellen Glutathion transient inWeitere Informationen
| Katalognummer | Menge |
|---|---|
| G36000 | 10 x 100 μg |
Katalognummer G36000
Preis (EUR)
476,00
Each
Menge:
10 x 100 μg
Preis (EUR)
476,00
Each
Biotinyliertes Glutathion-Ethylester (BioGEE) ist ein membrangängiges, biotinyliertes Glutathion-Analogon zum Nachweis von Glutathiolation. Unter oxidativem Stress können Zellen Glutathion transient in Proteine integrieren. Gestresste Zellen, die in BioGEE inkubiert werden, werden dieses biotinylierte Glutathion-Derivat auch in Proteine integrieren und so die Identifizierung oxidationsempfindlicher Proteine erleichtern. Sobald diese Zellen fixiert und permeabilisiert sind, können Sie die Glutathiolationswerte mit einem fluoreszierenden Streptavidin-Konjugat entweder mittels Durchflusszytometrie oder Fluoreszenzmikroskopie nachweisen. Proteine, die mit BioGEE glutathioliert werden, können auch mit Massenspektrometrie oder mit Western-Blotting-Methoden in Verbindung mit Fluorophor- oder enzymmarkierten Streptavidin-Konjugaten extrahiert und analysiert werden.
Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.
Specifications
Menge10 x 100 μg
ProdukttypGlutathione Reagent
Unit SizeEach
Zitierungen und Referenzen (15)
Zitierungen und Referenzen
Abstract
Glutathione directly reduces an oxidoreductase in the endoplasmic reticulum of mammalian cells.
Journal:J Biol Chem
PubMed ID:15507438
'The formation of disulfide bonds is an essential step in the folding of many glycoproteins and secretory proteins. Non-native disulfide bonds are often formed between incorrect cysteine residues, and thus the cell has dedicated a family of oxidoreductases that are thought to isomerize non-native bonds. For an oxidoreductase to be
S-glutathiolation by peroxynitrite of p21ras at cysteine-118 mediates its direct activation and downstream signaling in endothelial cells.
Journal:FASEB J
PubMed ID:16415107
'The highly reactive species, peroxynitrite, is produced in endothelial cells in pathological states in which the production of superoxide anion and NO is increased. Here, we show that peroxynitrite added exogenously or generated endogenously in response to exposure to an NO donor or oxidized low-density lipoproteins (oxLDL) increases p21ras activity
Detection and affinity purification of oxidant-sensitive proteins using biotinylated glutathione ethyl ester.
Journal:Methods Enzymol
PubMed ID:12078486
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Detecting oxidative post-translational modifications in proteins.
Journal:Amino Acids
PubMed ID:17021655
Oxidative stress induces various post-translational modifications (PTM); some are reversible in vivo via enzymatic catalysis. The present paper reviews specific procedures for the detection of oxidative PTM in proteins, most of them including electrophoresis. Main topics are carbonylated and glutathionylated proteins as well as modification of selected amino acids (Cys,
Glutathione binding to the Bcl-2 homology-3 domain groove: a molecular basis for Bcl-2 antioxidant function at mitochondria.
Journal:J Biol Chem
PubMed ID:17690097
Bcl-2 protects cells against mitochondrial oxidative stress and subsequent apoptosis. However, the mechanism underlying the antioxidant function of Bcl-2 is currently unknown. Recently, Bax and several Bcl-2 homology-3 domain (BH3)-only proteins (Bid, Puma, and Noxa) have been shown to induce a pro-oxidant state at mitochondria (1-4). Given the opposing effects