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Glutathione Ethyl Ester, Biotin Amide (BioGEE) (Glutathiolation Detection Reagent) - Special Packaging

Catalog number: G36000

Molecular Probes™  Related applications: Cell Viability, Proliferation & Function

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Description

Biotinylated glutathione ethyl ester (BioGEE) is a cell-permeant, biotinylated glutathione analog for the detection of glutathiolation. Under conditions of oxidative stress, cells may transiently incorporate glutathione into proteins. Stressed cells incubated in BioGEE will also incorporate this biotinylated glutathione derivative into proteins, facilitating the identification of oxidation-sensitive proteins. Once these cells are fixed and permeabilized, you can detect glutathiolation levels with a fluorescent streptavidin conjugate using either flow cytometry or fluorescence microscopy. Proteins glutathiolated with BioGEE can also be extracted and analyzed by mass spectrometry or by Western blotting methods in conjunction with fluorophore- or enzyme-labeled streptavidin conjugates.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Indicator Type: Free Radical Sensor
Indicator Properties: Protein-Based
Product Size: 10 x 100 µg
Shipping Condition: Room Temperature

Contents & storage

Store in freezer (-5 to -30°C).

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