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Invitrogen™

Glutathione Ethyl Ester, Biotin Amide (BioGEE) (Glutathiolation Detection Reagent) - Special Packaging

Catalog number:  G36000

Related applications:

Cell Analysis

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Description

Biotinylated glutathione ethyl ester (BioGEE) is a cell-permeant, biotinylated glutathione analog for the detection of glutathiolation. Under conditions of oxidative stress, cells may transiently incorporate glutathione into proteins. Stressed cells incubated in BioGEE will also incorporate this biotinylated glutathione derivative into proteins, facilitating the identification of oxidation-sensitive proteins. Once these cells are fixed and permeabilized, you can detect glutathiolation levels with a fluorescent streptavidin conjugate using either flow cytometry or fluorescence microscopy. Proteins glutathiolated with BioGEE can also be extracted and analyzed by mass spectrometry or by Western blotting methods in conjunction with fluorophore- or enzyme-labeled streptavidin conjugates.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Indicator: Free Radical Sensor
Indicator Properties: Protein-Based
Shipping Condition: Room Temperature

Contents & storage

Store in freezer (-5 to -30°C).