The FIX & PERM® Cell Permeabilization Kit is used in flow cytometry for fixing and permeabilizing cells in suspension, allowing access of antibody to intracellular targets without sacrificing scatter characteristics.
The FIX & PERM® Cell Permeabilization Kit is:
• Flexible—compatible with multiple sample types and cellular antigens • Robust—scatter pattern of cells unaffected • Efficient—simultaneous detection of intracellular and surface markers
The FIX & PERM® formula was specifically designed to reduce background staining and allow simultaneous addition of fluorochrome-labeled antibodies during the permeabilization step.The FIX & PERM® Cell Permeabilization Kit is suitable for various biological samples and has been validated for use with cell lines, whole blood, and bone marrow. The FIX & PERM® kit has been shown to accurately identify previously undetectable intracellular markers. Unlike other fixation and permeabilization reagents available on the market, the FIX & PERM® staining protocol leaves the morphological scatter characteristics of the cells intact.
How It Works The FIX & PERM® Cell Permeabilization Kit contains 2 reagents: Fixation Medium (Medium A) and Permeabilization Medium (Medium B). Cells are incubated for 15 minutes at room temperature with Fixation Medium, washed, and then incubated with the Permeabilization Medium and fluorochrome-labeled antibody. Fixation Medium and Permeabilization Medium are also available separately (Cat. Nos. GAS001S100 & GAS002S100).