Rat BCRP Vesicles

Catalog number: GM0007

Gibco™  Related applications: Target-Based ADME⁄Tox Assays

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

Rat BCRP Vesicles are insect derived purified plasma membranes with inserted BCRP transport protein (Breast Cancer Resistance Protein, ABCG2). Our Rat BCRP Vesicles can be used to evaluate test compounds and drug interactions with BCRP in in vitro assays. BSEP is an ATP-binding cassette (ABC) transporter associated with multidrug resistance, which carries various anti-cancer drugs such as topotecan, methotrexate and mitoxantrone.

Use Rat BCRP Vesicles to:

• Investigate the transporter interactions of your drug candidates
• Assess potential for transporter-mediated drug-drug interactions
• Obtain high quality results with a large signal to noise ratios

Concept Behind ABC Transporter Vesicles
ABC transporter vesicles are easy-to-use, efficient reagents for early assessment of a drug candidate's substrate and drug interaction potential. While ABC transporters typically mediate the export of substrates out of cells, transporters expressed on these inside-out vesicles import substrates into the vesicles. It is therefore possible to quantitatively evaluate transport activity for your compound by determining the amount incorporated into the vesicles.

Clear and Reliable Results
Prepared from Sf9 cells which have been engineered to over-express specific ABC transporters, these "inside-out" vesicles provide high levels of transporter activity with low background, giving you a clear signal if your compound is a substrate or inhibitor of a specific efflux transporter.

Transporter Vesicle Details
Rat BCRP Vesicles are part of a larger group of transporter vesicles known as ABC transporter vesicles. They are prepared from purified plasma membranes isolated from an insect cell system (Sf9 cells transfected with baculovirus) expressing BCRP. It is highly expressed in tumors like lung cancer and also in small intestinal epithelial cells, hepatic parenchymal cells, proximal renal tubule cells, and cerebrovascular endothelial cells. It is thought to be an important determinant of drug disposition, through its role in processes such as absorption at the small intestine, excretion into bile and urine, and transfer across the blood-brain barrier to the CNS.

For Research Use Only. Not for use in diagnostic procedures.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format: Vial(s)
Form: Frozen
Species: Rat
Product Size: 0.5 mL
Shipping Condition: Dry Ice
Purity or Quality Grade: Research Grade

Contents & storage

Store at -80°C.

Documents

Manuals & protocols

Recommended products