Invitrogen™

HCS LIVE/DEAD™ Green Kit

Catalog number: H10290
Invitrogen™

HCS LIVE/DEAD™ Green Kit

Catalog number: H10290
Catalog Number
H10290
Unit Size
1 kit
Price (USD)
392.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
H102901 kit
392.00
Your Price:
***
Product Overview
Figures
Videos
Documents
FAQ
Citations & References
Additional Information
Recommendations
The HCS LIVE⁄DEAD® Green Kit measures cytotoxicity by high content analysis with the Image-iT® DEAD Green™ viability stain – a non-fluorescent and cell impermeant compound that exhibits a strong fluorescent enhancement upon binding to DNA. Drugs and test compounds leading to serious cell injuries, including plasma membrane permeability, allow entry of the Image-iT® DEAD Green™ viability stain. This two-color fluorescence assay also employs the use of a cell permeant nuclear segmentation tool that stains both live and dead cells, reflecting the total cell number within the sample. For total cell staining, the kit includes both a blue-fluorescent Hoechst 33342 and a near infrared-fluorescent, HCS NuclearMask™ Deep Red reagent. Either of these segmentation tools may be used with Image-iT® DEAD Green™ viability stain for excellent discrimination of live and dead cells that survives fixation and permeabilization for combination with additional parameters of cytotoxicity or other biomarkers. The kit contains sufficient reagents for performing 2, 96-well plates.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Type
Eukaryotic Cells
Color
Green, Far-Red, Blue
Detection Method
Fluorescent
Dye Type
HCS NuclearMask™ Deep Red stain, Image-iT™ DEAD Green viability stain, Hoechst 33342
Emission
Visible, UV, near IR
Format
96-well plate
Product Line
LIVE/DEAD™, Molecular Probes™
Shipping Condition
Room Temperature
For Use With (Equipment)
Fluorescence Microscope

Contents & Storage

Store at ≤-20°C, desiccate and protect from light.

Figures

Frequently asked questions (FAQs)

Citations & References