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Citas y referencias (14)
Invitrogen™
HCS Mitochondrial Health Kit
El kit de salud mitocondrial de HCS mide simultánea y cuantitativamente dos parámetros importantes de salud celular: mitotoxicidad y citotoxicidad.Más información
| Número de catálogo | Cantidad |
|---|---|
| H10295 | 1 kit |
Número de catálogo H10295
Precio (MXN)
-
Cantidad:
1 kit
El kit de salud mitocondrial de HCS mide simultánea y cuantitativamente dos parámetros importantes de salud celular: mitotoxicidad y citotoxicidad. La tinción MitoHealth detecta cambios en el potencial de la membrana mitocondrial y, a continuación, el reactivo se acumula en las mitocondrias activas. El kit incluye Image-iT® DEAD™ Green para detectar la citotoxicidad, un tinte impermeable para las células sanas que se vuelve permeable cuando la membrana plasmática de las células se ve comprometida. Ambos indicadores son compatibles con la fijación y permeabilidad, permitiendo así el multiplexing con otros biomarcadores de interés. La herramienta de segmentación nuclear azul fluorescente Hoechst 33342 también se incluye y tiñe el ADN tanto en células vivas como muertas.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Método de detecciónFluorescente
Tipo de coloranteTinción de viabilidad Image-iT™ DEAD Green, tinción MitoHealth, Hoechst 33342
FormatoPlaca de 96 pocillos
Cantidad1 kit
Condiciones de envíoTemperatura ambiente
ColorNaranja, azul, verde
Para utilizar con (equipo)Microscopio de fluorescencia, Instrumentos de alto contenido
Línea de productosMolecular Probes
Tipo de productoKit de estado mitocondrial
Unit SizeEach
Contenido y almacenamiento
El kit contiene suficiente reactivo para procesar 2 placas de 96 pocillos.Almacenar a ≤- 20 °C, desecar y proteger de la luz.
Preguntas frecuentes
What is the fluorescence excitation/emission maxima of the stains in the HCS mitochondrial health kit?
Citations & References (14)
Citations & References
Abstract
Nullomer derived anticancer peptides (NulloPs): differential lethal effects on normal and cancer cells in vitro.
Journal:Peptides
PubMed ID:23000474
'We demonstrate the first use of the nullomer (absent sequences) approach to drug discovery and development. Nullomers are the shortest absent sequences determined in a species, or group of species. By identifying the shortest absent peptide sequences from the NCBI databases, we screened several potential anti-cancer peptides. In order to
High Content Analysis technology for evaluating the joint toxicity of sunset yellow and sodium sulfite in vitro.
Journal:Food Chem
PubMed ID:28530558
'Foods contain various additives that affect our daily lives. At present, food additive safety evaluation standards are based on the toxicity of single additives, but food additives are often used in combination and may have additive, synergistic or antagonistic actions. The current study investigated the toxicity of food additives and
Vitamin D regulates prostate cell metabolism via genomic and non-genomic mitochondrial redox-dependent mechanisms.
Journal:J Steroid Biochem Mol Biol
PubMed ID:31574299
'Vitamin D deficiency has been associated with increased risk for aggressive prostate cancer (PCa). Prostate epithelium has a unique metabolism compared to other tissues. Normal prostate exhibits low levels of mitochondrial respiration and there is a metabolic switch to increased oxidative phosphorylation in PCa. 25-hydroxyvitamin D (25(OH)D) is the major
Handling and Assessment of Human Primary Prostate Organoid Culture.
Journal:J Vis Exp
PubMed ID:30735176
'This paper describes a detailed protocol for three-dimensional (3D) culturing, handling, and evaluation of human primary prostate organoids. The process involves seeding of epithelial cells sparsely in a 3D matrix gel on a 96-well microplate with media changes to cultivate expansion into organoids. Morphology is then assessed by whole-well capturing
A high throughput imaging database of toxicological effects of nanomaterials tested on HepaRG cells.
Journal:Sci Data
PubMed ID:31048742
'The large amount of existing nanomaterials demands rapid and reliable methods for testing their potential toxicological effect on human health, preferably by means of relevant in vitro techniques in order to reduce testing on animals. Combining high throughput workflows with automated high content imaging techniques allows deriving much more information