Invitrogen™

HCS NuclearMask™ Stains

Catalog number: H10325
Have Questions?
Invitrogen™

HCS NuclearMask™ Stains

Catalog number: H10325
Have Questions?
Easily identify cell nuclei in high-content screening (HCS) assays with the versatile HCS NuclearMask Blue, Red, and Deep Red stains, which survive standard formaldehyde-based fixation and detergent-based permeabilization methods and can be applied to live and fixed cells. Because their dyes are easily discriminated from other fluorophores, the HCS NuclearMask stains can be used in multiparametric analyses. They can also label the entire cell (i.e., cytoplasm and nucleus) and provide an accurate backdrop against which to assess the features of interest.
 
Catalog Number
H10325
Unit Size
65 µL
Color
Blue
Price (USD)
Availability
-
Full specifications
Quantity
ColorBlue
Excitation Wavelength Range350/451
For Use With (Equipment)High Content Instrument
Quantity65 μL
Detection MethodFluorescence
DescriptionHCS NuclearMask™ Blue Stain
EmissionVisible
Product LineNuclearMask™
Shipping ConditionRoom Temperature
Label TypeFluorescent Dye
Product TypeStain
SubCellular LocalizationNucleic Acids, Nucleus
Unit Size65 µL
Catalog Number
Unit Size
125 µL
Color
Red
Price (USD)
Availability
-
Full specifications
Quantity
ColorRed
Excitation Wavelength Range622/645
For Use With (Equipment)High Content Instrument
Detection MethodFluorescence
DescriptionHCS NuclearMask™ Red Stain
EmissionVisible
Product LineNuclearMask™
Shipping ConditionRoom Temperature
Label TypeFluorescent Dye
Product TypeStain
SubCellular LocalizationNucleic Acids, Nucleus
Unit Size125 µL
Catalog Number
Unit Size
400 µL
Color
Far-Red, Deep Red
Price (USD)
Availability
-
Full specifications
Quantity
ColorFar-Red, Deep Red
Dye TypeHCS NuclearMask™ Deep Red stain
For Use With (Equipment)High Content Instrument
Volume (Metric)400 μL
Detection MethodFluorescence
DescriptionHCS NuclearMask™ Deep Red Stain (250X Concentrate in DMSO)
EmissionNear IR
Product LineNuclearMask™
Shipping ConditionRoom Temperature
Label TypeFluorescent Dye
Product TypeStain
SubCellular LocalizationNucleic Acids, Nucleus
Unit Size400 µL
Showing 3 of 3
Catalog NumberSpecificationsUnit SizeColorPrice (USD)AvailabilityQuantity
H10325Full specifications
65 µLBlue
-
ColorBlue
Excitation Wavelength Range350/451
For Use With (Equipment)High Content Instrument
Quantity65 μL
Detection MethodFluorescence
DescriptionHCS NuclearMask™ Blue Stain
EmissionVisible
Product LineNuclearMask™
Shipping ConditionRoom Temperature
Label TypeFluorescent Dye
Product TypeStain
SubCellular LocalizationNucleic Acids, Nucleus
Unit Size65 µL
H10326Full specifications
125 µLRed
-
ColorRed
Excitation Wavelength Range622/645
For Use With (Equipment)High Content Instrument
Detection MethodFluorescence
DescriptionHCS NuclearMask™ Red Stain
EmissionVisible
Product LineNuclearMask™
Shipping ConditionRoom Temperature
Label TypeFluorescent Dye
Product TypeStain
SubCellular LocalizationNucleic Acids, Nucleus
Unit Size125 µL
H10294Full specifications
400 µLFar-Red, Deep Red
-
ColorFar-Red, Deep Red
Dye TypeHCS NuclearMask™ Deep Red stain
For Use With (Equipment)High Content Instrument
Volume (Metric)400 μL
Detection MethodFluorescence
DescriptionHCS NuclearMask™ Deep Red Stain (250X Concentrate in DMSO)
EmissionNear IR
Product LineNuclearMask™
Shipping ConditionRoom Temperature
Label TypeFluorescent Dye
Product TypeStain
SubCellular LocalizationNucleic Acids, Nucleus
Unit Size400 µL
Showing 3 of 3
The versatile HCS NuclearMask Blue, HCS NuclearMask Red, and HCS NuclearMask Deep Red stains can be used to measure DNA content and perform cell demarcation with formaldehyde-fixed cells on high content imaging and analysis (HCS) platforms. HCS NuclearMask Blue, HCS NuclearMask Red, and HCS NuclarMask Deep Red stains have excitation/emission maxima of 355/460 nm, 622/645 nm, and 638/686 nm, respectively, when bound to DNA. HCS NuclearMask Blue stain is easily discriminated from visible- and near-IR wavelength fluorophores, HCS NuclearMask Red stain is easily discriminated from green- and even orange-fluorescent fluorophores, and HCS NuclearMask Deep Red is easily discriminated from visible wavelength fluorophores when used in multiparametric analyses.

If total cell staining is desired, HCS CellMask stains label the entire cell (cytoplasm and nucleus) and provide an accurate backdrop against which the features of interest can be assessed. HCS CellMask stains are available in a choice of colors for multiplexing flexibility. Sufficient volume quantities are provided to stain ten 96-well plates using an assay volume of 100 μL per well.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

Contents & Storage

Includes 65 μL of 2000X concentrate, providing sufficient material to stain a total of ten 96-well microplates.

Store at 2–6°C and protect from light.

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