Hoechst 33258 nucleic acid stain is a popular nuclear counterstain that emits blue fluorescence when bound to dsDNA. Its uses are similar to Hoechst 33342 (H-1399) for counterstaining, apoptosis and cell cycle studies, but Hoechst 33258 is reportedly less cell-permeant. This dye is also available in solution (H-3569).
For Research Use Only. Not for use in diagnostic procedures.
Excitation Wavelength Range
For Use With (Equipment)
Nucleus, Nucleic Acids
Contents & Storage
Store at room temperature and protect from light.
A zebrafish retina cryosection labeled with the mouse monoclonal antibody FRet 43 and detected using TSA Kit #2 with the HRP conjugate of goat anti–mouse IgG antibody and Alexa Fluor® 488 tyramide.
A J774 mouse macrophage cell stained with BODIPY® FL ganglioside GM1 (B13950) and Alexa Fluor® 555 dye–labeled cholera toxin subunit B.
Normal and camptothecin-treated bovine pulmondary artery endothelial cells.
Detection of BrdU-incorporating cells using the SBIP technique.
Live bovine pulmonary artery endothelial cells (BPAEC) stained with LysoTracker® Red DND-99, dihydrorhodamine 123 and Hoechst 33258.
In situ hybridization of a-satellite probes to human chromosomes 1, 15 and 17 detected by tyramide signal amplification.
Madin-Darby canine kidney (MDCK) cells.
Ovarioles from Drosophila melanogaster.
HeLa cell nuclei. Texas Red-X streptavidin, biotin-XX goat anti–mouse IgG antibody, ELF 97 Cytological Labeling Kit and Hoechst 33258.