Hoechst 33342 nucleic acid stain is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used to distinguish condensed pycnotic nuclei in apoptotic cells and for cell cycle studies in combination with BrdU. It is also available in solution (H-3570).
Osteosarcoma cells. Alexa Fluor® 488 concanavalin A, Alexa Fluor® 594 wheat germ agglutinin and Hoechst 33342 nucleic acid stain.
Nucleus and Golgi apparatus of a bovine pulmonary artery endothelial cell (BPAEC).
Bovine pulmonary artery endothelial cells stained with the reactive oxygen species indicator, 6-carboxy-2´,7´-dichlorodihydrofluorescein diacetate, di(acetoxymethyl ester) and FM® 5-95.
Endogenous phosphatase activity of osteoblast cells in a cartilaginous element of an adult zebrafish head localized using the ELF® 97 Endogenous Phosphatase Detection Kit.
Osteoblast cells in adult zebrafish head cryosection. ELF 97 Endogenous Phosphatase Detection Kit, Texas Red-X wheat germ agglutinin and Hoechst 33342 nucleic acid stain.
Live bovine pulmonary artery endothelial cells (BPAEC) labeled with LysoTracker® Red and Hoechst 33342.
Cultured axolotl sensory neurons stained with our anti–human neuronal protein HuC/HuD antibody and detected using the red-orange–fluorescent Alexa Fluor® 546 goat anti–mouse IgG antibody.
Bovine pulmonary artery endothelial cells. MitoFluor™ Far Red 680, LysoTracker® Green DND-26 and Hoechst 33342.
Centromeres of chromosome 1, chromosome 15 and chromosome 17.
Developing Drosophila embryo
Bull sperm. MitoTracker® Green FM and Hoechst 33342.
Detection of apoptosis in SK-N-MC neuroblastoma cells.
Photobleach protection using ProLong® Live Antifade Reagent
The ELF® 97 alcohol precipitate, demonstrating localization of endogenous alkaline phosphatase activity in the ciliary body of the zebrafish eye.
Nucleus and Golgi apparatus of a bovine pulmonary artery endothelial cell labeled with Hoechst 33342 and the BSA complex of BODIPY® FL C5-ceramide.
Human metaphase chromosome spreads.
Live NIH 3T3 cells labeled with probes for mitochondria, Golgi and the nucleus.
Human metaphase chromosomes hybridized to centromere probes labeled with ChromaTide OBEA-dCTP nucleotides.
Viable bovine pulmonary artery endothelial cells simultaneously stained with BODIPY® FL histamine and MitoTracker® Red CMXRos.
