Hoechst 33342 nucleic acid stain is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used to distinguish condensed pycnotic nuclei in apoptotic cells and for cell cycle studies in combination with BrdU. It is also available in solution (H-3570).
Stained and Fixed Neonatal Human Dermal Fibroblasts
Detection of apoptosis in SK-N-MC neuroblastoma cells.
Photobleach protection using ProLong® Live Antifade Reagent
Viable bovine pulmonary artery endothelial cells incubated simultaneously with MitoFluor™ Red 594 and Hoechst 33342.
Photobleach protection using ProLong® Live Antifade Reagent
Live NIH 3T3 cells labeled with probes for mitochondria, Golgi and the nucleus.
Human metaphase chromosomes hybridized to centromere probes labeled with ChromaTide OBEA-dCTP nucleotides.
Photobleach protection using ProLong® Live Antifade Reagent
Live J774 macrophage cells.
Cultured axolotl sensory neurons stained with our anti–human neuronal protein HuC/HuD antibody and detected using the red-orange–fluorescent Alexa Fluor® 546 goat anti–mouse IgG antibody.
Bovine pulmonary artery endothelial cells. MitoFluor™ Far Red 680, LysoTracker® Green DND-26 and Hoechst 33342.
Developing Drosophila embryo
The ELF® 97 alcohol precipitate, demonstrating localization of endogenous alkaline phosphatase activity in the ciliary body of the zebrafish eye.
Nucleus and Golgi apparatus of a bovine pulmonary artery endothelial cell (BPAEC).
Bovine pulmonary artery endothelial cells stained with the reactive oxygen species indicator, 6-carboxy-2´,7´-dichlorodihydrofluorescein diacetate, di(acetoxymethyl ester) and FM® 5-95.
Osteosarcoma cells. Alexa Fluor® 488 concanavalin A, Alexa Fluor® 594 wheat germ agglutinin and Hoechst 33342 nucleic acid stain.
Endogenous alkaline phosphatase activity of osteoblast cells in a cartilaginous element of an adult zebrafish head.
Endogenous phosphatase activity of osteoblast cells in a cartilaginous element of an adult zebrafish head localized using the ELF® 97 Endogenous Phosphatase Detection Kit.