Hoechst 33342 nucleic acid stain is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used to distinguish condensed pycnotic nuclei in apoptotic cells and for cell cycle studies in combination with BrdU. It is also available in solution (H-3570).

Also available as a room-temperature-stable, ready-to-use solution: NucBlue Live Cell Ready Probes Reagent.
See other Ready Probes ready-to-use imaging reagents and accessories ›

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Color

Blue

Excitation Wavelength Range

350⁄461

Dye Type

Hoechst 33342

For Use With (Equipment)

Fluorescence Microscope

Quantity

100mg

Detection Method

Fluorescent

Form

Solid

Shipping Condition

Room Temperature

Product Type

Nucleic Acid Stain

SubCellular Localization

Nucleus, Nucleic Acids

Contents & Storage

Store at room temperature and protect from light.

Figures

Endogenous alkaline phosphatase activity of osteosarcoma cells. ELF® 97 Endogenous Phosphatase Detection Kit and Hoechst 33342.

BPAE cells stained with Image-iT® LIVE Mitochondrial Transition Pore Assay Kit.

Zebrafish retina. ELF® 97 Immunohistochemistry Kit, Hoechst 33342 and tetramethylrhodamine wheat germ agglutinin.

Nucleus and Golgi apparatus of a bovine pulmonary artery endothelial cell labeled with Hoechst 33342 and the BSA complex of BODIPY® FL C5-ceramide.

Zebrafish retina. ELF® 97 Immunohistochemistry Kit, tetramethylrhodamine wheat germ agglutinin and Hoechst 33342

Viable bovine pulmonary artery endothelial cells simultaneously stained with BODIPY® FL histamine and MitoTracker® Red CMXRos.

Centromeres of chromosome 1, chromosome 15 and chromosome 17.

A mycoplasma-contaminated African green monkey fibroblast cell line stained with Hoechst 33342 nucleic acid stain.

Photobleach protection using ProLong® Live Antifade Reagent

Bovine pulmonary artery endothelial cells (BPAEC) stained with 6-carboxy-2',7'-dichlorodihydrofluorescein diacetate, FM 5-95 and Hoechst 33342.

Bull sperm. MitoTracker® Green FM and Hoechst 33342.

Live J774 macrophage cells labeled with BODIPY® FL C5-ganglioside GM1 and Alexa Fluor® 555 cholera toxin subunit B conjugate.

Endogenous alkaline phosphatase enzyme of osteosarcoma cells. Alexa Fluor® 594 goat anti–mouse IgG and Hoechst 33342.

Photobleach protection using ProLong® Live Antifade Reagent

Zebrafish retina. ELF® 97 Immunohistochemistry Kit, Hoechst 33342 and tetramethylrhodamine wheat germ agglutinin

Human metaphase chromosome spreads.

Osteoblast cells in adult zebrafish head cryosection. ELF 97 Endogenous Phosphatase Detection Kit, Texas Red-X wheat germ agglutinin and Hoechst 33342 nucleic acid stain.

Bovine pulmonary artery endothelial cells incubated simultaneously with CellTrace™ calcein red-orange AM and MitoTracker® Green FM.

Photobleach protection using ProLong® Live Antifade Reagent

Live bovine pulmonary artery endothelial cells (BPAEC) labeled with LysoTracker® Red and Hoechst 33342.

Endothelial cells.

Photobleach protection using ProLong® Live Antifade Reagent

Mouse Anti-Alpha Tubulin Monoclonal Antibody (Cat. No. A11126)

Stained and Fixed Neonatal Human Dermal Fibroblasts

Detection of apoptosis in SK-N-MC neuroblastoma cells.

Photobleach protection using ProLong® Live Antifade Reagent

Viable bovine pulmonary artery endothelial cells incubated simultaneously with MitoFluor™ Red 594 and Hoechst 33342.

Photobleach protection using ProLong® Live Antifade Reagent

Live NIH 3T3 cells labeled with probes for mitochondria, Golgi and the nucleus.

Human metaphase chromosomes hybridized to centromere probes labeled with ChromaTide OBEA-dCTP nucleotides.

Photobleach protection using ProLong® Live Antifade Reagent

Live J774 macrophage cells.

Cultured axolotl sensory neurons stained with our anti–human neuronal protein HuC/HuD antibody and detected using the red-orange–fluorescent Alexa Fluor® 546 goat anti–mouse IgG antibody.

Bovine pulmonary artery endothelial cells. MitoFluor™ Far Red 680, LysoTracker® Green DND-26 and Hoechst 33342.

Developing Drosophila embryo

The ELF® 97 alcohol precipitate, demonstrating localization of endogenous alkaline phosphatase activity in the ciliary body of the zebrafish eye.

Cultured axolotl sensory neurons. Anti–human neuronal protein HuC/HuD antibody, Alexa Fluor® 546 goat anti–mouse IgG antibody, Alexa Fluor® 488 phalloidin and Hoechst 33342.

Nucleus and Golgi apparatus of a bovine pulmonary artery endothelial cell (BPAEC).

Bovine pulmonary artery endothelial cells stained with the reactive oxygen species indicator, 6-carboxy-2´,7´-dichlorodihydrofluorescein diacetate, di(acetoxymethyl ester) and FM® 5-95.

Osteosarcoma cells. Alexa Fluor® 488 concanavalin A, Alexa Fluor® 594 wheat germ agglutinin and Hoechst 33342 nucleic acid stain.

Endogenous alkaline phosphatase activity of osteoblast cells in a cartilaginous element of an adult zebrafish head.

Endogenous phosphatase activity of osteoblast cells in a cartilaginous element of an adult zebrafish head localized using the ELF® 97 Endogenous Phosphatase Detection Kit.