Hoechst 33342, Trihydrochloride, Trihydrate, 100 mg
Description
Hoechst 33342 nucleic acid stain is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used to distinguish condensed pycnotic nuclei in apoptotic cells and for cell cycle studies in combination with BrdU. It is also available in solution (H-3570).Also available as a room-temperature-stable, ready-to-use solution: NucBlue Live Cell Ready Probes Reagent.
See other Ready Probes ready-to-use imaging reagents and accessories ›
Figures
Osteoblast cells in adult zebrafish head cryosection. ELF 97 Endogenous Phosphatase Detection Kit, Texas Red-X wheat germ agglutinin and Hoechst 33342 nucleic acid stain.
Endogenous alkaline phosphatase enzyme of osteosarcoma cells. Alexa Fluor® 594 goat anti–mouse IgG and Hoechst 33342.
Photobleach protection using ProLong® Live Antifade Reagent
Bovine pulmonary artery endothelial cells. MitoFluor™ Far Red 680, LysoTracker® Green DND-26 and Hoechst 33342.
Bull sperm. MitoTracker® Green FM and Hoechst 33342.
Human metaphase chromosome spreads.
Endogenous alkaline phosphatase activity of osteosarcoma cells. ELF® 97 Endogenous Phosphatase Detection Kit and Hoechst 33342.
J774 mouse macrophage cell.
Developing <i>Drosophila</i> embryo
Endogenous alkaline phosphatase activity of osteoblast cells in a cartilaginous element of an adult zebrafish head.
Nucleus and Golgi apparatus of a bovine pulmonary artery endothelial cell (BPAEC).
BPAE cells stained with Image-iT® LIVE Mitochondrial Transition Pore Assay Kit.
Viable bovine pulmonary artery endothelial cells simultaneously stained with BODIPY® FL histamine and MitoTracker® Red CMXRos.
A mycoplasma-contaminated African green monkey fibroblast cell line stained with Hoechst 33342 nucleic acid stain.
Cultured axolotl sensory neurons. Anti–human neuronal protein HuC/HuD antibody, Alexa Fluor® 546 goat anti–mouse IgG antibody, Alexa Fluor® 488 phalloidin and Hoechst 33342.
Mouse Anti-Alpha Tubulin Monoclonal Antibody (Cat. No. A11126)
Zebrafish retina. ELF® 97 Immunohistochemistry Kit, Hoechst 33342 and tetramethylrhodamine wheat germ agglutinin.
Stained and Fixed Neonatal Human Dermal Fibroblasts
Bovine pulmonary artery endothelial cells incubated simultaneously with CellTrace™ calcein red-orange AM and MitoTracker® Green FM.
Human metaphase chromosomes hybridized to centromere probes labeled with ChromaTide OBEA-dCTP nucleotides.
Live J774 macrophage cells.
Bovine pulmonary artery endothelial cells (BPAEC) stained with 6-carboxy-2',7'-dichlorodihydrofluorescein diacetate, FM 5-95 and Hoechst 33342.
Cultured axolotl sensory neurons stained with our anti–human neuronal protein HuC/HuD antibody and detected using the red-orange–fluorescent Alexa Fluor® 546 goat anti–mouse IgG antibody.
Endogenous phosphatase activity of osteoblast cells in a cartilaginous element of an adult zebrafish head localized using the ELF® 97 Endogenous Phosphatase Detection Kit.
Photobleach protection using ProLong® Live Antifade Reagent
Detection of apoptosis in SK-N-MC neuroblastoma cells.
The ELF® 97 alcohol precipitate, demonstrating localization of endogenous alkaline phosphatase activity in the ciliary body of the zebrafish eye.
Photobleach protection using ProLong® Live Antifade Reagent
Osteosarcoma cells. Alexa Fluor® 488 concanavalin A, Alexa Fluor® 594 wheat germ agglutinin and Hoechst 33342 nucleic acid stain.
Viable bovine pulmonary artery endothelial cells incubated simultaneously with MitoFluor™ Red 594 and Hoechst 33342.
Live NIH 3T3 cells labeled with probes for mitochondria, Golgi and the nucleus.
Live bovine pulmonary artery endothelial cells (BPAEC) labeled with LysoTracker® Red and Hoechst 33342.
Photobleach protection using ProLong® Live Antifade Reagent
Zebrafish retina. ELF® 97 Immunohistochemistry Kit, tetramethylrhodamine wheat germ agglutinin and Hoechst 33342
Photobleach protection using ProLong® Live Antifade Reagent
Centromeres of chromosome 1, chromosome 15 and chromosome 17.
Photobleach protection using ProLong® Live Antifade Reagent
Endothelial cells.
Bovine pulmonary artery endothelial cells stained with the reactive oxygen species indicator, 6-carboxy-2´,7´-dichlorodihydrofluorescein diacetate, di(acetoxymethyl ester) and FM® 5-95.
Zebrafish retina. ELF® 97 Immunohistochemistry Kit, Hoechst 33342 and tetramethylrhodamine wheat germ agglutinin
Nucleus and Golgi apparatus of a bovine pulmonary artery endothelial cell labeled with Hoechst 33342 and the BSA complex of BODIPY® FL C5-ceramide.
Live J774 macrophage cells labeled with BODIPY® FL C5-ganglioside GM1 and Alexa Fluor® 555 cholera toxin subunit B conjugate.
Photobleach protection using ProLong® Live Antifade Reagent
Specifications
| Excitation/Emission: | 350⁄461 |
|---|---|
| Form: | Solid |
| Label or Dye: | Hoechst 33342 |
| Shipping Condition: | Room Temperature |
| Sub Cellular Localization: | Nucleus, Nucleic Acids |
| Detection Method: | Fluorescent |
| For Use With (Equipment): | Fluorescence Microscope |
| Quantity: | 100mg |
| Color: | Blue |
| Cell Permeability: | Cell-Permeant |
