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Hypoxia Green Reagent for Flow Cytometry
Zitierungen und Referenzen (2)
Thermo Scientific™

Hypoxia Green Reagent for Flow Cytometry

Hypoxie-Grün-Reagenz für die Durchflusszytometrie ist ein antikörperunabhängiges Reagenz zum Nachweis niedriger Sauerstoffwerte in lebenden Zellen. Die membrandurchlässige Sonde setzt beiWeitere Informationen
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KatalognummerMenge
H200352 x 50 Assays
Katalognummer H20035
Preis (EUR)
322,65
Exklusiv online
430,00
Ersparnis 107,35 (25%)
Each
Menge:
2 x 50 Assays
Preis (EUR)
322,65
Exklusiv online
430,00
Ersparnis 107,35 (25%)
Each
Hypoxie-Grün-Reagenz für die Durchflusszytometrie ist ein antikörperunabhängiges Reagenz zum Nachweis niedriger Sauerstoffwerte in lebenden Zellen. Die membrandurchlässige Sonde setzt bei abnehmendem Sauerstoffgehalt Rhodamin frei, was zu einer fluorogenen Reaktion führt. Hypoxie ist ein Merkmal vieler Krankheiten, einschließlich kardiovaskulärer Erkrankungen und tumorvermittelter Immunsuppression, und ist entscheidend für das Überleben und Wachstum von Tumoren.

• Empfindliches, fixiertes Endpunkt-Assay
• Erkennt eine Abnahme des Sauerstoffs in lebenden Zellen
• Fluorogene, nicht antikörperbasierte Sonde

Specifications
NachweisverfahrenFluoreszent
FormatFläschchen
Menge2 x 50 Assays
VersandbedingungRaumtemperatur
Emission488
Zur Verwendung mit (Geräte)Durchflusszytometer
ProdukttypReagenz
Unit SizeEach
Inhalt und Lagerung
2 Fläschchen mit je 50 Tests
  • Bei -5 °C bis -30 °C lagern.

    • Häufig gestellte Fragen (FAQ)

    Can the Hypoxia Green Reagent for Flow Cytometry be used in cells expressing fluorescent proteins?

    Yes, as long as the fluorescent protein does not emit in the green range (˜510–535 nm). For cells that do not express a fluorescent protein, we recommend viewing unstained cells under the FITC channel to examine autofluorescence. For cells expressing a fluorescent protein, we recommend analyzing unstained cell samples to determine the extent the fluorescent protein emission may overlap in the FITC channel.

    Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

    Our Hypoxia Green Reagent for Flow Cytometry is already highly fluorescent before using the product. What could have caused this?

    This could be due to storage of the product under anaerobic or low oxygen conditions. The Hypoxia Green Reagent for Flow Cytometry increases in fluorescence upon exposure to low oxygen environments, and this change is not reversible. For some ROS indicators, we recommend storing the reagent under dry nitrogen or argon to prevent oxidation during storage. However, this is not appropriate for the Hypoxia Green Reagent for Flow Cytometry.

    Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

    What is different between the use of the Hypoxia Green Reagent for Flow Cytometry (Cat No. H20035) versus imaging using the Image-iT Green Hypoxia Reagent (Cat Nos. I14833, I14834)?

    The difference between these products is in the final working concentration of the reagent and incubation time. For flow cytometry applications, we recommend using a final concentration in the range of 0.5 to 1 µM with an incubation time ranging from 2 to 3 hrs. For imaging, we recommend a final concentration in the range of 1 to 10 µM with an incubation time ranging from 30 mins to 1 hr. For both applications, one should optimize the final working concentration and incubation time.

    Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

    Is Hypoxia Green Reagent for Flow Cytometry (Cat No. H20035) the same reagent as the Image-iT Green Hypoxia Reagent (Cat Nos. I14833, I14834)?

    The Hypoxia Green Reagent for Flow Cytometry and the Image-iT Green Hypoxia Reagent are the same reagent, formulated differently for flow cytometry and imaging use, respectively.

    Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

    Zitierungen und Referenzen (2)

    Zitierungen und Referenzen
    Abstract
    Catalase-loaded cisplatin-prodrug-constructed liposomes to overcome tumor hypoxia for enhanced chemo-radiotherapy of cancer.
    Authors:Zhang R, Song X, Liang C, Yi X, Song G, Chao Y, Yang Y, Yang K, Feng L, Liu Z
    Journal:Biomaterials
    PubMed ID:28550753
    Aiming at improved therapeutic efficacies, the combination of chemotherapy and radiotherapy (chemo-radiotherapy) has been widely studied and applied in clinic. However, the hostile characteristics of tumor microenvironment such as hypoxia often limit the efficacies in both types of cancer therapies. Herein, catalase (CAT), an antioxidant enzyme, is encapsulated inside liposomes ... More
    Tumor-targeted nanoplatform for in situ oxygenation-boosted immunogenic phototherapy of colorectal cancer.
    Authors:He H, Liu L, Liang R, Zhou H, Pan H, Zhang S, Cai L
    Journal:Acta Biomater
    PubMed ID:31945508
    Advanced colorectal cancer has a high mortality rate since conventional treatments have limited therapeutic effects and poor prognosis with high risks of metastasis and recurrence. Photodynamic therapy (PDT) is a promising treatment modality for the eradication of colorectal cancer, but its curative efficacy is severely affected by tumor hypoxia. Herein, ... More