Terrific Broth

Name: Terrific Broth
SKU: H26824.36

Terrific broth is a nutritionally rich medium for the growth of bacteria and used to promote strain maintenance, cloning, and protein expression.

Change view

Quantity:

100 g

500 g

Try the chem dex^TM web app

Catalog number H26824.36

also known as H26824-36

Price (USD)

Quantity:

500 g

Request bulk or custom format

Specifications

Packaging TypeGlass Bottle

Quantity500 g

Recommended StorageAmbient temperatures; Store under Nitrogen

SensitivityHygroscopic; Moisture sensitive

FormPowder

Terrific Broth (TB) is used with glycerol in cultivating recombinant strains of Escherichia coli. Terrific Broth II is a rich nutrient media for propagation of Escherichia coli for purposes of strain maintenance, cloning, protein expression. It is certified for plasmid and other bacterial cultures in liquid medium. Higher cell densities are achieved using Terrific Broth in comparison to traditional LB Broth.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General Description

• Terrific broth is a nutritional medium mainly constituted by peptone, yeast extract, glycerol (as a carbon source), amino acids, and water-soluble vitamins

Application

• Terrific broth is a highly enriched medium used with glycerol to cultivate bacteria, including Escherichia coli and recombinant strains

• Terrific broth medium is commonly used for maintainance of bacterial strains, for cloning, and for protein expression

• It is a certified medium for plasmid and other bacterial cultures in a liquid medium

• A higher cell density is obtained with this media compared to traditional LB broth

RUO – Research Use Only

General References:

Fidelibus, R. Peptide Expression and Purification to Elucidate the Mechanism of Vascular Alpha2C-Adrenoceptor Translocation. FASEB J. 2012, 26(8), 870.
Umechiyo, Tokumoto; Yasuhiro Takahashi. Genetic Analysis of the isc Operon in Escherichia coli Involved in the Biogenesis of Cellular Iron-Sulfur Proteins. Journal of biochemistry. 2001, 130 (1)