HCS LipidTOX™ Red Neutral Lipid Stain, for cellular imaging

Citations & References (16)

Invitrogen™

HCS LipidTOX™ Red Neutral Lipid Stain, for cellular imaging

中性脂肪の細胞内蓄積、すなわち脂肪変性は多くの場合、脂肪酸や中性脂肪の代謝に影響を与える薬物によって引き起こされます。HCS LipidTOX™ Red中性脂肪染色剤は、哺乳類細胞株において化合物が脂肪代謝に毒性作用を及ぼす可能性を特性評価する目的で開発されました。LipidTOX™中性脂肪染色剤は中性脂肪滴に対してきわめて親和性が高く、蛍光顕微鏡またはHCSリーダーで検出できます。このプローブは詳細を見る

製品番号（カタログ番号）	数量
H34476	各 1

製品番号（カタログ番号） H34476

価格（JPY）

66,900

お問い合わせください ›

中性脂肪の細胞内蓄積、すなわち脂肪変性は多くの場合、脂肪酸や中性脂肪の代謝に影響を与える薬物によって引き起こされます。HCS LipidTOX™ Red中性脂肪染色剤は、哺乳類細胞株において化合物が脂肪代謝に毒性作用を及ぼす可能性を特性評価する目的で開発されました。LipidTOX™中性脂肪染色剤は中性脂肪滴に対してきわめて親和性が高く、蛍光顕微鏡またはHCSリーダーで検出できます。このプローブは、HCS LipidTOX™リン脂質症検出試薬（H34350、H34351）に適合します。HCS LipidTOX™中性脂肪染色剤は、脂肪生成と呼ばれるプロセスである、脂肪細胞の形成および分化のモニタリングにも使用できます。

研究用にのみ使用できます。診断用には使用いただけません。

検出法蛍光

使用対象 (装置)ハイコンテント装置

製品ラインLipidTOX

数量各 1

出荷条件室温

標識タイプその他のラベルまたは色素

製品タイプ脂質染色

SubCellular LocalizationCell Membranes and Lipids , Lipids

Unit SizeEach

組成および保存条件

フリーザー（-5℃～-30℃）に保存し、遮光してください。

よくあるご質問（FAQ）

What kind of filter sets can I use with HCS LipidTOX neutral lipid stains?

LipidTOX Green neutral lipid stain can be imaged with filter sets appropriate for Alexa Fluor 488 dye or fluorescein. LipidTOX Red neutral lipid stain is best imaged with filter sets appropriate for Alexa Fluor 594 dye or Texas Red dye. LipidTOX Deep Red neutral lipid stain can imaged with filter sets appropriate for Alexa Fluor 647 dye or Cy5 dye.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to label the plasma membrane of my cells, but there are several dyes to choose from. Which one should I use?

For live-cell imaging, the CellVue and CellMask Plasma Membrane Stains are the most uniform and the slowest to be endocytosed. However, they are not the best choice if you wish to fix and permeabilize your cells, such as for antibody labeling. Wheat germ agglutinin (WGA) conjugates are also able to label live cells, or can label already formaldehyde-fixed cells. They can survive subsequent permeabilization with detergents, such as Triton X-100. If cells are already permeabilized, WGA will label internal structures as well. Thus, only an antibody against a plasma membrane protein can be used if cells are already permeabilized. Lipophilic cyanine dyes, such as DiI, will label all cell membranes in live cells, not just plasma membranes, if left on live cells for extended periods. Following page will help you choose (http://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-structure/plasma-membrane.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (16)

引用および参考文献

Abstract

Vascular lipid accumulation, lipoprotein oxidation, and macrophage lipid uptake in hypercholesterolemic zebrafish.

Authors:Stoletov K, Fang L, Choi SH, Hartvigsen K, Hansen LF, Hall C, Pattison J, Juliano J, Miller ER, Almazan F, Crosier P, Witztum JL, Klemke RL, Miller YI,

Journal:Circ Res

PubMed ID:19265037

'Lipid accumulation in arteries induces vascular inflammation and atherosclerosis, the major cause of heart attack and stroke in humans. Extreme hyperlipidemia induced in mice and rabbits enables modeling many aspects of human atherosclerosis, but microscopic examination of plaques is possible only postmortem. Here we report that feeding adult zebrafish (Danio ... More

Enhancement of BODIPY505/515 lipid fluorescence method for applications in biofuel-directed microalgae production.

Authors:Brennan L, Blanco Fernández A, Mostaert AS, Owende P,

Journal:J Microbiol Methods

PubMed ID:22521923

'This paper describes a microalgal cell lipid fluorescence enhancement method using BODIPY(505/515), which can be used to screen for lipids in wild-type microalgae and to monitor lipid content within microalgae production processes to determine optimal harvesting time. The study was based on four microalgae species (Dunaliella teteriolecta, Tetraselmis suecica, Nannochloropsis ... More

Increased lipid accumulation in the Chlamydomonas reinhardtii sta7-10 starchless isoamylase mutant and increased carbohydrate synthesis in complemented strains.

Authors:Work VH, Radakovits R, Jinkerson RE, Meuser JE, Elliott LG, Vinyard DJ, Laurens LM, Dismukes GC, Posewitz MC,

Journal:Eukaryot Cell

PubMed ID:20562225

'The accumulation of bioenergy carriers was assessed in two starchless mutants of Chlamydomonas reinhardtii (the sta6 [ADP-glucose pyrophosphorylase] and sta7-10 [isoamylase] mutants), a control strain (CC124), and two complemented strains of the sta7-10 mutant. The results indicate that the genetic blockage of starch synthesis in the sta6 and sta7-10 mutants ... More

Knockdown of ACAT-1 reduces amyloidogenic processing of APP.

Authors:Huttunen HJ, Greco C, Kovacs DM

Journal:FEBS Lett

PubMed ID:17412327

'Previous studies have shown that acyl-coenzyme A:cholesterol acyl transferase (ACAT), an enzyme that controls cellular equilibrium between free cholesterol and cholesteryl esters, modulates proteolytic processing of APP in cell-based and animal models of Alzheimer''s disease. Here we report that ACAT-1 RNAi reduced cellular ACAT-1 protein by approximately 50% and cholesteryl ... More

Microplate-based high throughput screening procedure for the isolation of lipid-rich marine microalgae.

Authors:Pereira H, Barreira L, Mozes A, Florindo C, Polo C, Duarte CV, Custódio L, Varela J,

Journal:Biotechnol Biofuels

PubMed ID:22192119

We describe a new selection method based on BODIPY (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene) staining, fluorescence activated cell sorting (FACS) and microplate-based isolation of lipid-rich microalgae from an environmental sample. Our results show that direct sorting onto solid medium upon FACS can save about 3 weeks during the scale-up process as compared with the ... More

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