Functional analysis of FSP27 protein regions for lipid droplet localization, caspase-dependent apoptosis, and dimerization with CIDEA.
AuthorsLiu K, Zhou S, Kim JY, Tillison K, Majors D, Rearick D, Lee JH, Fernandez-Boyanapalli RF, Barricklow K, Houston MS, Smas CM,
JournalAm J Physiol Endocrinol Metab
PubMed ID19843876
'The adipocyte-specific protein FSP27, also known as CIDEC, is one of three cell death-inducing DFF45-like effector (CIDE) proteins. The first known function for CIDEs was promotion of apoptosis upon ectopic expression in mammalian cells. Recent studies in endogenous settings demonstrated key roles for CIDEs in energy metabolism. FSP27 is a ... More
Functional Interactions between Mldp (LSDP5) and Abhd5 in the Control of Intracellular Lipid Accumulation.
AuthorsGranneman JG, Moore HP, Mottillo EP, Zhu Z,
JournalJ Biol Chem
PubMed ID19064991
'Cellular lipid metabolism is regulated in part by protein-protein interactions near the surface of intracellular lipid droplets. This work investigated functional interactions between Abhd5, a protein activator of the lipase Atgl, and Mldp, a lipid droplet scaffold protein that is highly expressed in oxidative tissues. Abhd5 was highly targeted to ... More
Fluorescent high-content imaging allows the discrimination and quantitation of E-LDL-induced lipid droplets and Ox-LDL-generated phospholipidosis in human macrophages.
AuthorsGrandl M, Schmitz G,
JournalCytometry A
PubMed ID20014301
'Macrophage foam cells formed during uptake of atherogenic lipoproteins are a hallmark of atherosclerotic lesion development. In this study, human macrophages were incubated with two prototypic atherogenic LDL modifications enzymatically degraded LDL (E-LDL) and oxidized LDL (Ox-LDL) prepared from the same donor LDL. To detect differences in macrophage lipid storage, ... More
Enhancement of BODIPY505/515 lipid fluorescence method for applications in biofuel-directed microalgae production.
AuthorsBrennan L, Blanco Fernández A, Mostaert AS, Owende P,
JournalJ Microbiol Methods
PubMed ID22521923
'This paper describes a microalgal cell lipid fluorescence enhancement method using BODIPY(505/515), which can be used to screen for lipids in wild-type microalgae and to monitor lipid content within microalgae production processes to determine optimal harvesting time. The study was based on four microalgae species (Dunaliella teteriolecta, Tetraselmis suecica, Nannochloropsis ... More
Increased lipid accumulation in the Chlamydomonas reinhardtii sta7-10 starchless isoamylase mutant and increased carbohydrate synthesis in complemented strains.
AuthorsWork VH, Radakovits R, Jinkerson RE, Meuser JE, Elliott LG, Vinyard DJ, Laurens LM, Dismukes GC, Posewitz MC,
JournalEukaryot Cell
PubMed ID20562225
'The accumulation of bioenergy carriers was assessed in two starchless mutants of Chlamydomonas reinhardtii (the sta6 [ADP-glucose pyrophosphorylase] and sta7-10 [isoamylase] mutants), a control strain (CC124), and two complemented strains of the sta7-10 mutant. The results indicate that the genetic blockage of starch synthesis in the sta6 and sta7-10 mutants ... More
Microplate-based high throughput screening procedure for the isolation of lipid-rich marine microalgae.
AuthorsPereira H, Barreira L, Mozes A, Florindo C, Polo C, Duarte CV, Custódio L, Varela J,
JournalBiotechnol Biofuels
PubMed ID22192119
We describe a new selection method based on BODIPY (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene) staining, fluorescence activated cell sorting (FACS) and microplate-based isolation of lipid-rich microalgae from an environmental sample. Our results show that direct sorting onto solid medium upon FACS can save about 3 weeks during the scale-up process as compared with the ... More
In situ analysis of heterogeneity in the lipid content of single green microalgae in alginate hydrogel microcapsules.
AuthorsLee DH, Bae CY, Han JI, Park JK,
Journal
PubMed ID24007509
Microalgae, a group of microorganisms that grow using sunlight as the sole energy source and carbon dioxide as an only carbon source, have been considered as a feedstock of choice for the production of biofuels such as biodiesel. To explore the economic feasibility of such application, however, many technical hurdles ... More
NS3 helicase domains involved in infectious intracellular hepatitis C virus particle assembly.
AuthorsMa Y, Yates J, Liang Y, Lemon SM, Yi M,
JournalJ Virol
PubMed ID18508894
A mutation within subdomain 1 of the hepatitis C virus (HCV) NS3 helicase (NS3-Q221L) (M. Yi, Y. Ma, J. Yates, and S. M. Lemon, J. Virol. 81:629-638, 2007) rescues a defect in production of infectious virus by an intergenotypic chimeric RNA (HJ3). Although NS3-Gln-221 is highly conserved across HCV genotypes, ... More
Obesity Shapes Metabolism in the Tumor Microenvironment to Suppress Anti-Tumor Immunity.
Authors
JournalCell
PubMed ID33301708
Partitioning of MLX-Family Transcription Factors to Lipid Droplets Regulates Metabolic Gene Expression.
Authors
JournalMol Cell
PubMed ID32023484
Caspase-1 activity in microglia stimulated by pro-inflammagen nanocrystals.
Authors
JournalACS Nano
PubMed ID24107183
A Wnt-mediated transformation of the bone marrow stromal cell identity orchestrates skeletal regeneration.
Authors
JournalNat Commun
PubMed ID31949165
Sympathetic neuron-associated macrophages contribute to obesity by importing and metabolizing norepinephrine.
Authors
JournalNat Med
PubMed ID29035364
Ring finger protein 213 assembles into a sensor for ISGylated proteins with antimicrobial activity.
Authors
JournalNat Commun
PubMed ID34599178
PLCγ1 suppression promotes the adaptation of KRAS-mutant lung adenocarcinomas to hypoxia.
Authors
JournalNat Cell Biol
PubMed ID33077911
CHP1 Regulates Compartmentalized Glycerolipid Synthesis by Activating GPAT4.
Authors
JournalMol Cell
PubMed ID30846317
A subset of chondrogenic cells provides early mesenchymal progenitors in growing bones.
Authors
JournalNat Cell Biol
PubMed ID25419849
Omega-3 Fatty Acids Activate Ciliary FFAR4 to Control Adipogenesis.