Hoechst 33342, Trihydrochloride, Trihydrate - 10 mg/mL Solution in Water
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Description
Hoechst 33342 nucleic acid stain is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used to distinguish condensed pycnotic nuclei in apoptotic cells and for cell-cycle studies in combination with BrdU.Figures
Fluorescent probes generated with ARES™ DNA Labeling Kits hybridized to human metaphase chromosome spreads.
Photobleach protection using ProLong® Live Antifade Reagent
Hoechst 33342.
Human metaphase chromosomes hybridized to centromere probes labeled with ChromaTide® OBEA-dCTP nucleotides.
Detection of apoptosis in SK-N-MC neuroblastoma cells.
Zebrafish retina. ELF® 97 Immunohistochemistry Kit, tetramethylrhodamine wheat germ agglutinin and Hoechst 33342
Photobleach protection using ProLong® Live Antifade Reagent
Human metaphase chromosome spreads.
Photobleach protection using ProLong® Live Antifade Reagent
Endogenous phosphatase activity of osteoblast cells in a cartilaginous element of an adult zebrafish head localized using the ELF® 97 Endogenous Phosphatase Detection Kit.
Viable bovine pulmonary artery endothelial cells simultaneously stained with BODIPY® FL histamine and MitoTracker® Red CMXRos.
Bovine pulmonary artery endothelial cells incubated simultaneously with CellTrace™ calcein red-orange AM and MitoTracker® Green FM.
Fluorescent probes generated with ARES™ DNA Labeling Kits hybridized to human metaphase chromosome spreads.
Osteoblast cells in adult zebrafish head cryosection. ELF 97 Endogenous Phosphatase Detection Kit, Texas Red-X wheat germ agglutinin and Hoechst 33342 nucleic acid stain.
Bovine pulmonary artery endothelial cells (BPAEC) stained with 6-carboxy-2',7'-dichlorodihydrofluorescein diacetate, FM 5-95 and Hoechst 33342.
Bovine pulmonary artery endothelial cells. MitoFluor™ Far Red 680, LysoTracker® Green DND-26 and Hoechst 33342.
Endogenous alkaline phosphatase activity of osteoblast cells in a cartilaginous element of an adult zebrafish head.
Live J774 macrophage cells labeled with BODIPY® FL C5-ganglioside GM1 and Alexa Fluor® 555 cholera toxin subunit B conjugate.
Photobleach protection using ProLong® Live Antifade Reagent
Photobleach protection using ProLong® Live Antifade Reagent
Viable bovine pulmonary artery endothelial cells incubated simultaneously with MitoFluor™ Red 594 and Hoechst 33342.
Stained and Fixed Neonatal Human Dermal Fibroblasts
Human metaphase chromosomes hybridized to centromere probes labeled with ChromaTide OBEA-dCTP nucleotides.
BPAE cells stained with Image-iT® LIVE Mitochondrial Transition Pore Assay Kit.
U-2 OS cells were transduced with Cellular Lights™ Actin-RFP (Cat. No. C10127) and Cellular Lights™ MAP4-GFP (Cat. No. C10105) and imaged the next day.
Zebrafish retina. ELF® 97 Immunohistochemistry Kit, Hoechst 33342 and tetramethylrhodamine wheat germ agglutinin.
Photobleach protection using ProLong® Live Antifade Reagent
J774 mouse macrophage cell.
Osteosarcoma cells. Alexa Fluor® 488 concanavalin A, Alexa Fluor® 594 wheat germ agglutinin and Hoechst 33342 nucleic acid stain.
Bovine pulmonary artery endothelial cells stained with the reactive oxygen species indicator, 6-carboxy-2´,7´-dichlorodihydrofluorescein diacetate, di(acetoxymethyl ester) and FM® 5-95.
Zebrafish retina. ELF® 97 Immunohistochemistry Kit, Hoechst 33342 and tetramethylrhodamine wheat germ agglutinin
Bull sperm. MitoTracker® Green FM and Hoechst 33342.
Live J774 macrophage cells.
Cultured axolotl sensory neurons stained with our anti–human neuronal protein HuC/HuD antibody and detected using the red-orange–fluorescent Alexa Fluor® 546 goat anti–mouse IgG antibody.
Centromeres of chromosome 1, chromosome 15 and chromosome 17.
Live-cell imaging with Organelle Lights™ Talin-RFP and Organelle Lights™ Mito-GFP in HeLa cells. HeLa cell were transduced with Cellular Lights™ Talin-RFP and Organelle Lights™ Mito-GFP and co-stained with 1 mg/mL Hoechst 33342. Imaging was performed on live cells using a Delta Vision Core microscope and standard DAPI/FITC/TRITC filter sets.
A mycoplasma-contaminated African green monkey fibroblast cell line stained with Hoechst 33342 nucleic acid stain.
Live NIH 3T3 cells labeled with probes for mitochondria, Golgi and the nucleus.
Photobleach protection using ProLong® Live Antifade Reagent
Specifications
| Color: | Blue |
|---|---|
| For Use With (Equipment): | Fluorescence Microscope |
| Cell Permeability: | Cell-Permeant |
| Sub-Cellular Localization: | Nucleic Acids, Nucleus |
| Detection Method: | Fluorescent |
| Excitation⁄Emission (nm): | 350⁄461 |
| Form: | Liquid |
| Label or Dye: | Hoechst 33342 |
| Product Size: | 10 mL |
| Shipping Condition: | Room Temperature |
