Hoechst 33342, Trihydrochloride, Trihydrate - 10 mg/mL Solution in Water
Description
Hoechst 33342 nucleic acid stain is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used to distinguish condensed pycnotic nuclei in apoptotic cells and for cell-cycle studies in combination with BrdU.Also available as a room-temperature-stable, ready-to-use solution: NucBlue Live Cell Ready Probes Reagent.
See other Ready Probes ready-to-use imaging reagents and accessories ›
Figures
Photobleach protection using ProLong® Live Antifade Reagent
Cultured axolotl sensory neurons stained with our anti–human neuronal protein HuC/HuD antibody and detected using the red-orange–fluorescent Alexa Fluor® 546 goat anti–mouse IgG antibody.
Bovine pulmonary artery endothelial cells stained with the reactive oxygen species indicator, 6-carboxy-2´,7´-dichlorodihydrofluorescein diacetate, di(acetoxymethyl ester) and FM® 5-95.
Bovine pulmonary artery endothelial cells (BPAEC) stained with 6-carboxy-2',7'-dichlorodihydrofluorescein diacetate, FM 5-95 and Hoechst 33342.
Osteosarcoma cells. Alexa Fluor® 488 concanavalin A, Alexa Fluor® 594 wheat germ agglutinin and Hoechst 33342 nucleic acid stain.
Live J774 macrophage cells.
Photobleach protection using ProLong® Live Antifade Reagent
Photobleach protection using ProLong® Live Antifade Reagent
Photobleach protection using ProLong® Live Antifade Reagent
Detection of apoptosis in SK-N-MC neuroblastoma cells.
Zebrafish retina. ELF® 97 Immunohistochemistry Kit, Hoechst 33342 and tetramethylrhodamine wheat germ agglutinin
Endogenous phosphatase activity of osteoblast cells in a cartilaginous element of an adult zebrafish head localized using the ELF® 97 Endogenous Phosphatase Detection Kit.
Live NIH 3T3 cells labeled with probes for mitochondria, Golgi and the nucleus.
Bovine pulmonary artery endothelial cells. MitoFluor™ Far Red 680, LysoTracker® Green DND-26 and Hoechst 33342.
Photobleach protection using ProLong® Live Antifade Reagent
Zebrafish retina. ELF® 97 Immunohistochemistry Kit, Hoechst 33342 and tetramethylrhodamine wheat germ agglutinin.
Human metaphase chromosome spreads.
J774 mouse macrophage cell.
Viable bovine pulmonary artery endothelial cells incubated simultaneously with MitoFluor™ Red 594 and Hoechst 33342.
Viable bovine pulmonary artery endothelial cells simultaneously stained with BODIPY® FL histamine and MitoTracker® Red CMXRos.
Zebrafish retina. ELF® 97 Immunohistochemistry Kit, tetramethylrhodamine wheat germ agglutinin and Hoechst 33342
Bull sperm. MitoTracker® Green FM and Hoechst 33342.
BPAE cells stained with Image-iT® LIVE Mitochondrial Transition Pore Assay Kit.
Human metaphase chromosomes hybridized to centromere probes labeled with ChromaTide OBEA-dCTP nucleotides.
Live-cell imaging with Organelle Lights™ Talin-RFP and Organelle Lights™ Mito-GFP in HeLa cells. HeLa cell were transduced with Cellular Lights™ Talin-RFP and Organelle Lights™ Mito-GFP and co-stained with 1 mg/mL Hoechst 33342. Imaging was performed on live cells using a Delta Vision Core microscope and standard DAPI/FITC/TRITC filter sets.
Endogenous alkaline phosphatase activity of osteoblast cells in a cartilaginous element of an adult zebrafish head.
Osteoblast cells in adult zebrafish head cryosection. ELF 97 Endogenous Phosphatase Detection Kit, Texas Red-X wheat germ agglutinin and Hoechst 33342 nucleic acid stain.
U-2 OS cells were transduced with Cellular Lights™ Actin-RFP (Cat. No. C10127) and Cellular Lights™ MAP4-GFP (Cat. No. C10105) and imaged the next day.
Photobleach protection using ProLong® Live Antifade Reagent
Centromeres of chromosome 1, chromosome 15 and chromosome 17.
A mycoplasma-contaminated African green monkey fibroblast cell line stained with Hoechst 33342 nucleic acid stain.
Human metaphase chromosomes hybridized to centromere probes labeled with ChromaTide® OBEA-dCTP nucleotides.
Stained and Fixed Neonatal Human Dermal Fibroblasts
Hoechst 33342.
Live J774 macrophage cells labeled with BODIPY® FL C5-ganglioside GM1 and Alexa Fluor® 555 cholera toxin subunit B conjugate.
Fluorescent probes generated with ARES™ DNA Labeling Kits hybridized to human metaphase chromosome spreads.
Photobleach protection using ProLong® Live Antifade Reagent
Bovine pulmonary artery endothelial cells incubated simultaneously with CellTrace™ calcein red-orange AM and MitoTracker® Green FM.
Specifications
| Excitation/Emission: | 350⁄461 |
|---|---|
| Form: | Liquid |
| Label or Dye: | Hoechst 33342 |
| Shipping Condition: | Room Temperature |
| Sub Cellular Localization: | Nucleus, Nucleic Acids |
| Detection Method: | Fluorescent |
| For Use With (Equipment): | Fluorescence Microscope |
| Quantity: | 10mL |
| Color: | Blue |
| Cell Permeability: | Cell-Permeant |
