Measuring reactive species and oxidative damage in vivo and in cell culture: how should you do it and what do the results mean?
AuthorsHalliwell B, Whiteman M
JournalBr J Pharmacol
PubMed ID15155533
'Free radicals and other reactive species (RS) are thought to play an important role in many human diseases. Establishing their precise role requires the ability to measure them and the oxidative damage that they cause. This article first reviews what is meant by the terms free radical, RS, antioxidant, oxidative ... More
Externally applied pressure activates pancreatic stellate cells through the generation of intracellular reactive oxygen species.
AuthorsAsaumi H, Watanabe S, Taguchi M, Tashiro M, Otsuki M,
JournalAm J Physiol Gastrointest Liver Physiol
PubMed ID17761838
'Local tissue pressure is higher in chronic pancreatitis than in the normal pancreas. We reported recently that pressure application induces synthesis of extracellular matrix (ECM) and cytokines in pancreatic stellate cells (PSCs) and that epigallocatechin gallate (EGCG), a potent antioxidant, inhibits the transformation of PSCs from quiescent to activated phenotype ... More
The detection and quantification of highly reactive oxygen species using the novel HPF fluorescence probe in a rat model of focal cerebral ischemia.
AuthorsTomizawa S, Imai H, Tsukada S, Simizu T, Honda F, Nakamura M, Nagano T, Urano Y, Matsuoka Y, Fukasaku N, Saito N
JournalNeurosci Res
PubMed ID16168507
'A novel fluorescence probe, 2-[6-(4''-hydroxy) phenoxy-3H-xanthen-3-on-9-yl] benzoic acid (HPF) was used to investigate the generation of highly reactive oxygen species (hROS) under ischemia both in vitro and in vivo. In the in vitro study, HT 22 cells were used to demonstrate that was predominantly detected in the cytoplasm, which coincides ... More
Time to fatigue is increased in mouse muscle at 37 degrees C; the role of iron and reactive oxygen species.
AuthorsReardon TF, Allen DG,
JournalJ Physiol
PubMed ID19470779
'Studies exploring the rate of fatigue in isolated muscle at 37 degrees C have produced mixed results. In the present study, muscle fibre bundles from the mouse foot were used to study the effect of temperature on the rate of muscle fatigue. Provided iron was excluded from the solutions, time ... More
Fluorescence probes used for detection of reactive oxygen species.
AuthorsGomes A, Fernandes E, Lima JL,
JournalJ Biochem Biophys Methods
PubMed ID16297980
'Endogenously produced pro-oxidant reactive species are essential to life, being involved in several biological functions. However, when overproduced (e.g. due to exogenous stimulation), or when the levels of antioxidants become severely depleted, these reactive species become highly harmful, causing oxidative stress through the oxidation of biomolecules, leading to cellular damage ... More
Mechanism of action of sensors for reactive oxygen species based on fluorescein-phenol coupling: the case of 2-[6-(4'-hydroxy)phenoxy-3H-xanthen-3-on-9-yl]benzoic acid.
AuthorsHeyne B, Maurel V, Scaiano JC,
JournalOrg Biomol Chem
PubMed ID16493462
'We demonstrate the ability of a sensor containing a tethered fluorescein-phenol structure to react with peroxyl radicals and with an oxidizing agent such as potassium ferricyanide. This latter reaction yields the corresponding peroxyl radical as observed by EPR analysis. We propose that the reaction of the sensor with peroxyl and ... More
Monitoring singlet oxygen and hydroxyl radical formation with fluorescent probes during photodynamic therapy.
AuthorsPrice M, Reiners JJ, Santiago AM, Kessel D,
JournalPhotochem Photobiol
PubMed ID19508643
'Singlet oxygen (1O2) is the primary oxidant generated in photodynamic therapy (PDT) protocols involving sensitizers resulting in type II reactions. 1O2 can give rise to additional reactive oxygen species (ROS) such as the hydroxyl radical (*OH). The current study was designed to assess 3''-p-(aminophenyl) fluorescein (APF) and 3''-p-(hydroxyphenyl) fluorescein (HPF) ... More
Myeloperoxidase is a key regulator of oxidative stress mediated apoptosis in myeloid leukemic cells.
AuthorsNakazato T, Sagawa M, Yamato K, Xian M, Yamamoto T, Suematsu M, Ikeda Y, Kizaki M,
JournalClin Cancer Res
PubMed ID17875773
'PURPOSE: We reported previously that reactive oxygen species (ROS) are key mediators of apoptosis induced by a polyphenol, (-)-epigallocatechin-3-gallate (EGCG), in myeloid leukemic cells. This study aimed to further examine the mechanism of ROS-mediated apoptosis induced by EGCG and its relationship to the heme enzyme myeloperoxidase (MPO). EXPERIMENTAL DESIGN: We ... More
Evidence of ROS generation by mitochondria in cells with impaired electron transport chain and mitochondrial DNA damage.
AuthorsIndo HP, Davidson M, Yen HC, Suenaga S, Tomita K, Nishii T, Higuchi M, Koga Y, Ozawa T, Majima HJ,
JournalMitochondrion
PubMed ID17307400
'Mitochondrial damage is a well known cause of mitochondria-related diseases. A major mechanism underlying the development of mitochondria-related diseases is thought to be an increase in intracellular oxidative stress produced by impairment of the mitochondrial electron transport chain (ETC). However, clear evidence of intracellular free radical generation has not been ... More
Fluorescent and luminescent probes for measurement of oxidative and nitrosative species in cells and tissues: progress, pitfalls, and prospects.
AuthorsWardman P,
JournalFree Radic Biol Med
PubMed ID17761297
Chemical probes for free radicals in biology are important tools; fluorescence and chemiluminescence offer high detection sensitivity. This article reviews progress in the development of probes for "reactive oxygen and nitrogen" species, emphasizing the caution needed in their use. Reactive species include hydrogen peroxide; hydroxyl, superoxide, and thiyl radicals; carbonate ... More
Hydrogen-rich saline attenuates radiation-induced male germ cell loss in mice through reducing hydroxyl radicals.
AuthorsChuai Y, Gao F, Li B, Zhao L, Qian L, Cao F, Wang L, Sun X, Cui J, Cai J,
JournalBiochem J
PubMed ID22077489
Our recent studies suggest that H2 (hydrogen) has a potential as a novel radioprotector without known toxic side effects. The present study was designed to examine the underlying radioprotective mechanism of H2 and its protective role on irradiated germ cells. Produced by the Fenton reaction and radiolysis of H2O, hydroxyl ... More
A common mechanism of cellular death induced by bactericidal antibiotics.
AuthorsKohanski MA, Dwyer DJ, Hayete B, Lawrence CA, Collins JJ,
JournalCell
PubMed ID17803904
Antibiotic mode-of-action classification is based upon drug-target interaction and whether the resultant inhibition of cellular function is lethal to bacteria. Here we show that the three major classes of bactericidal antibiotics, regardless of drug-target interaction, stimulate the production of highly deleterious hydroxyl radicals in Gram-negative and Gram-positive bacteria, which ultimately ... More
Glutathione depletion is necessary for apoptosis in lymphoid cells independent of reactive oxygen species formation.
AuthorsFranco R, Panayiotidis MI, Cidlowski JA,
JournalJ Biol Chem
PubMed ID17724027
Changes in the intracellular redox environment of cells have been reported to be critical for the activation of apoptotic enzymes and the progression of programmed cell death. Glutathione (GSH) depletion is an early hallmark observed in apoptosis, and we have demonstrated that GSH efflux during death receptor-mediated apoptosis occurs via ... More
Development of novel fluorescence probes that can reliably detect reactive oxygen species and distinguish specific species.
AuthorsSetsukinai K, Urano Y, Kakinuma K, Majima HJ, Nagano T
JournalJ Biol Chem
PubMed ID12419811
We designed and synthesized 2-[6-(4'-hydroxy)phenoxy-3H-xanthen-3-on-9-yl]benzoic acid (HPF) and 2- [6-(4'-amino)phenoxy-3H-xanthen-3-on-9-yl]benzoic acid (APF) as novel fluorescence probes to detect selectively highly reactive oxygen species (hROS) such as hydroxyl radical (*OH) and reactive intermediates of peroxidase. Although HPF and APF themselves scarcely fluoresced, APF selectively and dose-dependently afforded a strongly fluorescent compound, ... More
Reproductive protein protects functionally sterile honey bee workers from oxidative stress.
AuthorsSeehuus SC, Norberg K, Gimsa U, Krekling T, Amdam GV
JournalProc Natl Acad Sci U S A
PubMed ID16418279
Research on aging shows that regulatory pathways of fertility and senescence are closely interlinked. However, evolutionary theories on social species propose that lifelong care for offspring can shape the course of senescence beyond the restricted context of reproductive capability. These observations suggest that control circuits of aging are remodeled in ... More
A ruthenium(ii) based photosensitizer and transferrin complexes enhance photo-physical properties, cell uptake, and photodynamic therapy safety and efficacy.
AuthorsKaspler P, Lazic S, Forward S, Arenas Y, Mandel A, Lilge L
JournalPhotochem Photobiol Sci
PubMed ID26947517
'Metal-based photosensitizers are of interest as their absorption and chemical binding properties can be modified via the use of different ligands. Ru(2+) based photosensitizers are known to be effective photodynamic therapy (PDT) agents against bacteria, whereas use for oncological indications in vivo has not been demonstrated with the same level ... More
No evident dose-response relationship between cellular ROS level and its cytotoxicity--a paradoxical issue in ROS-based cancer therapy.
Authors
JournalSci Rep
PubMed ID24848642
Photoinduced electron-transfer mechanisms for radical-enhanced photodynamic therapy mediated by water-soluble decacationic C₇₀ and C₈₄O₂ Fullerene Derivatives.
Authors
JournalNanomedicine
PubMed ID23117043
Free Radical-Mediated Protein Radical Formation in Differentiating Monocytes.
Authors
JournalInt J Mol Sci
PubMed ID34576127
Exploring the Antimicrobial Action of Quaternary Amines against Acinetobacter baumannii.
Authors
JournalMBio
PubMed ID29437928
Dps Protects Enterohemorrhagic Escherichia coli against Acid-Induced Antimicrobial Peptide Killing.
Authors
JournalJ Bacteriol
PubMed ID32229529
Nickel-induced epithelial-mesenchymal transition by reactive oxygen species generation and E-cadherin promoter hypermethylation.
Authors
JournalJ Biol Chem
PubMed ID22648416
A Short Peptide Derived from the ZorO Toxin Functions as an Effective Antimicrobial.
Authors
JournalToxins (Basel)
PubMed ID31277504
Glutamine sensitivity analysis identifies the xCT antiporter as a common triple-negative breast tumor therapeutic target.
Authors
JournalCancer Cell
PubMed ID24094812
Low-level laser therapy stimulates the oxidative burst in human neutrophils and increases their fungicidal capacity.
AuthorsCerdeira CD, Lima Brigagão MR, Carli ML, de Souza Ferreira C, de Oliveira Isac Moraes G, Hadad H, Costa Hanemann JA, Hamblin MR, Sperandio FF
JournalJ Biophotonics
PubMed ID27243910
Low-level laser therapy (LLLT) is known to enhance mitochondrial electron transfer and ATP production; thus, this study asked whether LLLT could stimulate the oxidative burst in human neutrophils (PMN) and improve their ability to kill microorganisms. Blood from healthy human subjects was collected and PMN were isolated from the samples. ... More
Photodynamic therapy for Schistosoma mansoni: Promising outcomes.
Authorsde Melo NB, Dos Santos LFM, de Castro MS, Souza RLM, Marques MJ, Castro AP, de Castro AT, de Carli ML, Hanemann JAC, Silva MS, Moraes GOI, Beijo LA, Brigagão MRPL, Sperandio FF
JournalJ Photochem Photobiol B
PubMed ID29024873
The purpose of this study was to assess, for the very first time, the effects of photodynamic therapy (PDT) on Schistosoma mansoni in vitro by measuring reactive oxygen species (ROS) generation throughout the treatment, as well as the behavior of the parasites (mating, motility and contraction/shortening), and damage to their ... More
An Integrin-Targeted, Highly Diffusive Construct for Photodynamic Therapy.
Targeted antineoplastic agents show great promise in the treatment of cancer, having the ability to impart cytotoxicity only to specific tumor types. However, these therapies do not experience uniform uptake throughout tumors, leading to sub-lethal cell killing that can impart treatment resistance, and cause problematic off-target effects. Here we demonstrate ... More
Photodynamic inactivation of Paracoccidioides brasiliensis helps the outcome of oral paracoccidiodomycosis.
AuthorsDos Santos LFM, Melo NB, de Carli ML, Mendes ACSC, Bani GMAC, Verinaud LM, Burger E, de Oliveira I Moraes G, Pereira AAC, Brigagão MRL, Hanemann JAC, Sperandio FF
JournalLasers Med Sci
PubMed ID28349345
The antifungal drug therapy often employed to treat paracoccidiodomycosis (PCM), an important neglected fungal systemic infection, leads to offensive adverse effects, besides being very long-lasting. In addition, PCM compromises the oral health of patients by leading to oral lesions that are very painful and disabling. In that way, photodynamic therapy ... More
Aldose reductase inhibitor, fidarestat prevents doxorubicin-induced endothelial cell death and dysfunction.
AuthorsSonowal H, Pal P, Shukla K, Saxena A, Srivastava SK, Ramana KV
JournalBiochem Pharmacol
PubMed ID29458045
Despite doxorubicin (Dox) being one of the most widely used chemotherapy agents for breast, blood and lung cancers, its use in colon cancer is limited due to increased drug resistance and severe cardiotoxic side effects that increase mortality associated with its use at high doses. Therefore, better adjuvant therapies are ... More
Chidamide-induced ROS accumulation and miR-129-3p-dependent cell cycle arrest in non-small lung cancer cells.
AuthorsWu YF, Ou CC, Chien PJ, Chang HY, Ko JL, Wang BY
JournalPhytomedicine
PubMed ID30668358
Epigenetic therapy is a promising popular treatment modality for various cancers. Histone modification and miRNA should not be underestimated in lung cancer. This study aimed to investigate whether chidamide, a histone deacetylase inhibitor (HDACi), which inhibits telomerase activity and induces cell cycle arrest, influences ROS and miRNA production in non-small ... More