Fetal Hemoglobin Test Kit, FITC

Catalog number:  HFH01

 Related applications:

Flow Cytometry

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The Fetal Hemoglobin (HbF) Test is based on a monoclonal antibody directed to fetal hemoglobin (hemoglobin F). This antibody is conjugated with fluorescein (FITC). Commonly used, the fluorescein conjugates provide relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility.

This reagent is intended for the identification followed by enumeration of fetal red blood cells. Fetal cells are identified by the presence of fetal hemoglobin using a flow cytometric method. The Fetal Hemoglobin Test may be used as an aid in identifying fetal-maternal hemorrhage and determining the need for immunoprophylaxis with immune globulin.

Antibody Specifications


Fetal Hemoglobin F (HbF), mouse IgG1 monoclonal


Fluorescein (FITC)

Validated Application:

Flow Cytometry

Species Reactivity:


Ex/Em and laser configuration:

488/519 nm, blue laser

Clone ID:


Regulatory Status:


Antibody characteristics include:
• Clinical relevance
in vitro diagonstic

Clinical Relevance
The Fetal Hemoglobin Test contains a monoclonal antibody directed to HbF that specifically recognizes fetal hemoglobin found in fetal erythrocytes. The test permits the enumeration of fetal erythrocytes in the maternal circulation as a result of a fetal-maternal hemorrhage, or leakage, of fetal cells into the maternal circulation. Hemorrhage of cells from an Rh+ fetus into the circulation of an Rh- mother may result in the formation of Rh-reactive antibodies in the mother. Rh hemolytic anemia in a subsequent Rh+ fetus may result from placental transfer of antibodies formed in the mother to the fetus. Although the volume of fetal erythrocytes found in the maternal circulation during pregnancy and immediately post-partum is reported to be small and of uncertain clinical significance in many cases, substantial hemorrhage may result from a number of causes including fetal or maternal trauma and placental defects.

Erythrocytes containing fetal hemoglobin may be found in individuals of any age, but with lower amounts of fetal hemoglobin compared to fetal red cells. These cells have been termed F cells. High levels of F cells may also exist in adults with a heterogeneous group of genetic disorders of uncertain etiology, referred to as Hereditary Persistence of Fetal Hemoglobin. Other clinical conditions causing significant levels of anemia may also result in elevated levels of F cells. Several clinical conditions have been described with increased levels of F cells. These conditions include hereditary anemic diseases such as sickle cell anemia and thalassemia major.

In Vitro Diagnostic
For In Vitro Diagnostic Use.

Related Links
Use the Spectraviewer to help you plan your experiments. Additional general information about Life Technologies flow cytometry products and support is also available.
For In Vitro Diagnostic Use. For Research Use Only in China.


Reactivity: Human
Host Species: Mouse
SubClass (Isotype): IgG1
Label or Dye: FITC (Fluorescein)
Number of Reactions: 100 Tests
Product Size: 100 tests
Shipping Condition: Wet Ice

Contents & storage

Store in refrigerator (2–8°C).


Manuals & protocols